Miyagi University of Education, Sendai, Miyagi, Japan.
Sendai Daisan High School, Sendai, Miyagi, Japan.
Methods Mol Biol. 2021;2219:31-48. doi: 10.1007/978-1-0716-0974-3_2.
Pseudopotamilla occelata is a polychaete worm distributed widely in the northern part of the Pacific coast, having value as fishing bait as well as biological material for some basic research areas, including reproduction. Here we describe methods for handling the gametes and embryos of this worm, focusing on such topics as maintenance of adults, induction of oocyte maturation and fertilization, culture of embryos and larvae, microinjection into oocytes, and calcium (Ca) imaging.
拟沼虾虎鱼是一种广泛分布于太平洋北部的多毛类环节蠕虫，可用作鱼饵，也可用作某些基础研究领域的生物材料，包括繁殖。在这里，我们描述了处理这种蠕虫配子和胚胎的方法，重点介绍了成体的维持、卵母细胞成熟和受精的诱导、胚胎和幼虫的培养、卵母细胞的微注射以及钙离子（Ca）成像等主题。
