Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL, USA.
Department of Biochemistry and Molecular Genetics, Midwestern University, Glendale, AZ, USA.
Methods Mol Biol. 2021;2219:151-161. doi: 10.1007/978-1-0716-0974-3_9.
To provide a better understanding of the composition of the egg cell membrane, we describe a method in which proteins and peptides that are either naturally released by the egg or cleaved by sperm proteases can be collected, analyzed, and identified. Such molecules are captured and isolated from the surrounding seawater via biotinylation, before being concentrated by an affinity interaction and subsequently analyzed by western blotting and mass spectrometry.
为了更好地了解卵细胞膜的组成，我们描述了一种方法，可以收集、分析和鉴定自然释放的卵子蛋白和肽或由精子蛋白酶切割的蛋白和肽。这些分子通过生物素化从周围海水中捕获和分离，然后通过亲和相互作用浓缩，随后通过 Western blot 和质谱进行分析。
