Department of Zoology, Moinul Hoque Choudhury Memorial Science College, Algapur, Assam, India.
Department of Biotechnology, Assam University, Silchar, Assam, India.
Arch Insect Biochem Physiol. 2021 Jan;106(1):e21750. doi: 10.1002/arch.21750. Epub 2020 Oct 19.
To understand the synonymous codon usage pattern in mitochondrial genome of Antheraea assamensis, we analyzed the 13 mitochondrial protein-coding genes of this species using a bioinformatic approach as no work was reported yet. The nucleotide composition analysis suggested that the percentages of A, T, G,and C were 33.73, 46.39, 9.7 and 10.17, respectively and the overall GC content was 19.86, that is, lower than 50% and the genes were AT rich. The mean effective number of codons of mitochondrial protein-coding genes was 36.30 and it indicated low codon usage bias (CUB). Relative synonymous codon usage analysis suggested overrepresented and underrepresented codons in each gene and the pattern of codon usage was different among genes. Neutrality plot analysis revealed a narrow range of distribution for GC content at the third codon position and some points were diagonally distributed, suggesting both mutation pressure and natural selection influenced the CUB.
为了理解阿萨姆蚕线粒体基因组中同义密码子的使用模式，我们采用生物信息学方法分析了该物种的 13 个线粒体蛋白编码基因，因为目前还没有相关工作的报道。核苷酸组成分析表明，A、T、G 和 C 的百分比分别为 33.73%、46.39%、9.7%和 10.17%，总 GC 含量为 19.86%，即低于 50%，且基因富含 AT。线粒体蛋白编码基因的平均有效密码子数为 36.30，表明密码子使用偏性较低（CUB）。相对同义密码子使用分析表明，每个基因中都有代表性过高和过低的密码子，并且基因之间的密码子使用模式也不同。中性绘图分析显示，第三密码子位置的 GC 含量分布范围较窄，有些点呈对角线分布，表明突变压力和自然选择都对 CUB 产生了影响。
