Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, 300387, Tianjin, China.
Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, 300387, Tianjin, China.
Aquat Toxicol. 2020 Dec;229:105579. doi: 10.1016/j.aquatox.2020.105579. Epub 2020 Sep 30.
Effective phytoremediation by aquatic plant such as duckweed could be applied to solve Cd pollution. In the present study, the impact of Graphene oxide (GO) on the accumulation of Cd in duckweed has been studied. The response of duckweed was also investigated, concluding growth, Cd flux, and gene expression response. Results showed that GO promoted the accumulation of Cd in duckweed. After 6 h of Cd enrichment in duckweed, Cd content was about 1.4 times that of the control group at fronds and 1.25 times that of the control group at roots, meanwhile, Cd content in the water system was 0.67 times that of the control group. The Cd influx increased significantly. 4471 genes were up-regulated and 3230 genes were down-regulated significantly as duckweed treated with GO under Cd treatment. Moreover, phagosome pathway was downregulated, some key proteins: Stx7, Rab7 and Tubastatin B (TUBB) were significantly downregulated with GO addition under Cd stress. Scanning electron microscope (SEM) observation showed that GO and Cd were attached on the cell surface of duckweed as white crystal. GO could be applied in phytoremediation by duckweed of Cd in aquatic system.
水生植物如浮萍的有效植物修复可以应用于解决 Cd 污染问题。本研究研究了氧化石墨烯（GO）对浮萍积累 Cd 的影响。还研究了浮萍的反应，包括生长、Cd 通量和基因表达反应。结果表明，GO 促进了浮萍中 Cd 的积累。在浮萍中富集 Cd 6 小时后，叶片中的 Cd 含量约为对照组的 1.4 倍，根中的 Cd 含量约为对照组的 1.25 倍，同时，水系统中的 Cd 含量为对照组的 0.67 倍。Cd 流入量显著增加。在 Cd 处理下，浮萍用 GO 处理后，有 4471 个基因显著上调，3230 个基因显著下调。此外，吞噬体途径下调，一些关键蛋白：Stx7、Rab7 和 Tubastatin B（TUBB）在 Cd 胁迫下添加 GO 后显著下调。扫描电子显微镜（SEM）观察表明，GO 和 Cd 附着在浮萍细胞表面，呈白色晶体。GO 可应用于水生系统中浮萍对 Cd 的植物修复。
