采用二维气相色谱飞行时间质谱法比较液液萃取和搅拌棒吸附萃取法对废水中多类有机污染物的萃取效果。
A comparison of liquid-liquid extraction and stir bar sorptive extraction for multiclass organic contaminants in wastewater by comprehensive two-dimensional gas chromatography time of flight mass spectrometry.
机构信息
Department of Chemistry, The Pennsylvania State University, University Park, PA, USA.
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA.
出版信息
Talanta. 2021 Jan 1;221:121481. doi: 10.1016/j.talanta.2020.121481. Epub 2020 Aug 6.
Liquid-liquid extraction (LLE) and stir bar sorptive extraction (SBSE) are extraction methods used for the analysis of contaminants in aqueous samples. In this study, both LLE and SBSE were compared for the extraction of priority pollutants and contaminants of emerging concern (CECs) in wastewater influent and effluent samples, for analysis with comprehensive two-dimensional gas chromatography with time of flight mass spectrometry (GC × GC-TOFMS). The methods were compared for their extraction efficiency of a broad range of compounds, matrix effects, accurate and reliable quantification of targets, and sensitivity. The target analytes studied were semi-volatile organic compounds (SVOC) including polycyclic aromatic hydrocarbons, phenols, phthalate esters, anilines, ethers, aromatic nitro compounds, and nitrosamines. LLE allowed for a higher number of target analytes to be extracted with over 70% recovery and quantified more targets in the influent samples. Matrix interference effects had a negative impact on the recovery of non-polar contaminants, such as polycyclic aromatic hydrocarbons (PAHs), in the influent water samples especially with SBSE. In SBSE, 24 target analytes demonstrated significant matrix interference leading to poor analyte recovery and 13 analytes were negatively affected in the same way in LLE. Generally, polar compounds also demonstrated poor extraction with SBSE in both effluent and influent water samples. However, SBSE effluent chromatograms contained about three times as many total analytes as compared with LLE, suggesting that SBSE is more sensitive for trace contaminants in effluent samples. Based on this research, LLE is recommended for studies seeking to quantify a broad range of target analytes in complex matrices, like wastewater influent. SBSE is an appropriate method for the non-target and survey analysis of trace contaminants in less complex water samples.
液液萃取（LLE）和搅拌棒吸附萃取（SBSE）是用于分析水样中污染物的萃取方法。在这项研究中，比较了 LLE 和 SBSE 两种方法在废水进水和出水样品中优先污染物和新兴关注污染物（CECs）的萃取效果，并用全二维气相色谱飞行时间质谱联用（GC×GC-TOFMS）进行分析。比较了两种方法对多种化合物的萃取效率、基质效应、目标物的准确可靠定量和灵敏度。研究的目标分析物包括半挥发性有机化合物（SVOC），包括多环芳烃、酚类、邻苯二甲酸酯、苯胺、醚类、芳香族硝基化合物和亚硝胺。LLE 允许提取更多的目标分析物，回收率超过 70%，并能定量更多的进水样品中的目标物。基质干扰效应对非极性污染物（如多环芳烃）的回收率有负面影响，特别是在进水水样中用 SBSE 萃取时。在 SBSE 中，有 24 个目标分析物表现出显著的基质干扰，导致分析物回收率低，而 13 个分析物在 LLE 中也受到同样的影响。一般来说，极性化合物在废水和进水水样中用 SBSE 萃取效果也较差。然而，SBSE 出水色谱图中总分析物的数量比 LLE 多约三倍，这表明 SBSE 对废水样品中痕量污染物更敏感。基于这项研究，LLE 推荐用于研究复杂基质如废水进水时，定量分析广泛的目标分析物。SBSE 是一种适用于非靶向和痕量污染物调查分析的方法，适用于较简单的水样。
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