Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
Talanta. 2021 Jan 1;221:121668. doi: 10.1016/j.talanta.2020.121668. Epub 2020 Sep 15.
Rapid and sensitive diagnosis of bacterial infections at early stage is of great significance for food safety monitoring as well as clinical treatment. Herein, we construct a surface-enhanced Raman scattering (SERS) nanoprobe based on M13 phages for the selective detection and inactivation of Staphylococcus aureus (S. aureus). M13 phage with specific S. aureus-binding heptapeptide displayed on the N-terminal of pIII protein is selected from phage display peptide library. The S. aureus-specific SERS probe is thus constructed by in situ growth of gold nanoparticles (AuNPs) on M13 phage surface, followed by modification with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) as SERS active molecule. Upon the addition of this SERS probe, M13 phage selectively binds with S. aureus to induce anchoring of AuNPs on S. aureus surface, and the SERS probe-labeled S. aureus cells are collected by centrifugation for SERS detection. For the quantification of S. aureus, a linear range of 10-10 cfu mL is achieved in aqueous medium. It is further demonstrated by spiking recovery in soft drinks. Furthermore, this SERS probe exhibits bactericidal capabilities towards S. aureus, which shows promising potential to serve as a multifunctional platform for simultaneous detection and inactivation of S. aureus.
快速、灵敏地诊断细菌感染在食品安全监测和临床治疗中具有重要意义。在此，我们构建了一种基于 M13 噬菌体的表面增强拉曼散射（SERS）纳米探针，用于选择性检测和杀伤金黄色葡萄球菌（S. aureus）。从噬菌体展示肽库中筛选出具有特定 S. aureus 结合七肽的 M13 噬菌体，该七肽位于 pIII 蛋白的 N 端。S. aureus 特异性 SERS 探针通过在 M13 噬菌体表面原位生长金纳米粒子（AuNPs），然后用 5,5-二硫代双（2-硝基苯甲酸）（DTNB）进行修饰来构建，作为 SERS 活性分子。加入该 SERS 探针后，M13 噬菌体选择性地与 S. aureus 结合，诱导 AuNPs 在 S. aureus 表面锚定，SERS 探针标记的 S. aureus 细胞通过离心收集进行 SERS 检测。在水相介质中，S. aureus 的定量范围为 10-10 cfu mL-1，呈线性关系。在软饮料中添加回收物进行验证。此外，该 SERS 探针对 S. aureus 具有杀菌能力，有望成为一种多功能平台，用于同时检测和杀伤 S. aureus。
