Oike Takahiro, Hirota Yuka, Dewi Maulany Darwis Narisa, Shibata Atsushi, Ohno Tatsuya
Department of Radiation Oncology, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi 371-8511, Japan.
Heavy Ion Medical Center, Gunma University, 3-39-22, Showa-machi, Maebashi 371-8511, Japan.
J Pers Med. 2020 Oct 15;10(4):171. doi: 10.3390/jpm10040171.
Clonogenic assays are the gold standard to measure in vitro radiosensitivity, which use two cell plating methods, before or after irradiation (IR). However, the effect of the plating method on the experimental outcome remains unelucidated. By using common cancer cell lines, here we demonstrate that pre-IR and post-IR plating methods have a negligible effect on the clonogenic assay-derived photon sensitivity as assessed by SF, SF, SF, SF, D, or D (N.B. SFx indicates the survival at X Gy; Dx indicates the dose providing X% survival). These data provide important biological insight that supports inter-study comparison and integrated analysis of published clonogenic assay data regardless of the plating method used.
克隆形成试验是测量体外放射敏感性的金标准，该试验在照射（IR）之前或之后使用两种细胞接种方法。然而，接种方法对实验结果的影响仍未阐明。通过使用常见的癌细胞系，我们在此证明，如通过SF、SF、SF、SF、D或D所评估的，照射前和照射后接种方法对克隆形成试验得出的光子敏感性影响可忽略不计（注意，SFx表示在X Gy时的存活率；Dx表示提供X%存活率的剂量）。这些数据提供了重要的生物学见解，支持了无论使用何种接种方法，对已发表的克隆形成试验数据进行研究间比较和综合分析。
