Institute of Pathology, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia.
Department of Abdominal Surgery, University Medical Center Ljubljana, Zaloška cesta 2, 1000 Ljubljana, Slovenia.
Epigenomics. 2020 Oct;12(19):1677-1688. doi: 10.2217/epi-2020-0118. Epub 2020 Oct 20.
Identification of aberrant hypermethylation in promoter regions of candidate genes to discover potential biomarkers for colorectal cancer. Genes , , , , , and were pre-selected in a bioinformatics study for their hypermethylation status in colorectal cancer. Methylation analysis was performed on 202 cancer tissue specimens to validate candidate genes. Genes and displayed methylation in 95.3 and 99.7% of The Cancer Genome Atlas dataset samples and in 96 and 98% of our experimentally tested samples, respectively. and promoter hypermethylation holds diagnostic biomarker potential in patients with early colorectal cancer.
鉴定候选基因启动子区域的异常高甲基化，以发现结直肠癌潜在的生物标志物。在生物信息学研究中，针对这些基因在结直肠癌中的高甲基化状态进行了预筛选。对 202 例癌组织标本进行了甲基化分析，以验证候选基因。在癌症基因组图谱数据集样本中，基因和分别有 95.3%和 99.7%显示出甲基化，在我们实验测试的样本中分别有 96%和 98%显示出甲基化。和启动子高甲基化在早期结直肠癌患者中具有诊断生物标志物的潜力。
