W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States of America.
The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, United States of America.
PLoS Negl Trop Dis. 2020 Oct 20;14(10):e0008729. doi: 10.1371/journal.pntd.0008729. eCollection 2020 Oct.
The yellow fever mosquito Aedes aegypti is a prolific vector of arboviral and filarial diseases that largely relies on its sense of smell to find humans. To facilitate in-depth analysis of the neural circuitry underlying Ae. aegypti olfactory-driven behaviors, we generated an updated in vitro atlas for the antennal lobe olfactory brain region of this disease vector using two independent neuronal staining methods. We performed morphological reconstructions with replicate fixed, dissected and stained brain samples from adult male and female Ae. aegypti of the LVPib12 genome reference strain and determined that the antennal lobe in both sexes is comprised of approximately 80 discrete glomeruli. Guided by landmark features in the antennal lobe, we found 63 of these glomeruli are stereotypically located in spatially invariant positions within these in vitro preparations. A posteriorly positioned, mediodorsal glomerulus denoted MD1 was identified as the largest spatially invariant glomerulus in the antennal lobe. Spatial organization of glomeruli in a recently field-derived strain of Ae. aegypti from Puerto Rico was conserved, despite differences in antennal lobe shape relative to the inbred LVPib12 strain. This model in vitro atlas will serve as a useful community resource to improve antennal lobe annotation and anatomically map projection patterns of neurons expressing target genes in this olfactory center. It will also facilitate the development of chemotopic maps of odor representation in the mosquito antennal lobe to decode the molecular and cellular basis of Ae. aegypti attraction to human scent and other chemosensory cues.
黄热病蚊埃及伊蚊是多种虫媒病毒和丝虫病的高效传播媒介，主要依赖嗅觉寻找人类。为了深入分析埃及伊蚊嗅觉驱动行为的神经回路，我们使用两种独立的神经元染色方法，为这种病媒的触角叶嗅觉大脑区域生成了一个更新的体外图谱。我们对来自 LVPib12 基因组参考株的成年雄性和雌性埃及伊蚊的重复固定、解剖和染色脑样本进行了形态重建，确定两性的触角叶由大约 80 个离散的神经节构成。在触角叶的标志性特征的指导下，我们发现这些神经节中有 63 个在这些体外制剂中以空间不变的位置典型地定位。一个位于后位的中背侧神经节，称为 MD1，被确定为触角叶中最大的空间不变神经节。尽管与近交系 LVPib12 菌株相比，触角叶的形状存在差异，但来自波多黎各的最近野外衍生的埃及伊蚊菌株的神经节空间组织是保守的。这个体外图谱模型将作为一个有用的社区资源，用于改进触角叶的注释，并对表达目标基因的神经元在这个嗅觉中心的解剖投射模式进行定位。它还将有助于解码埃及伊蚊对人体气味和其他化学感觉线索的吸引力的分子和细胞基础，以绘制蚊子触角叶中气味表示的趋化地图。
