State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Sciences and Technology, Anhui Agricultural University, Hefei, China; Anhui Academy of Medical Science, Hefei, China.
State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Sciences and Technology, Anhui Agricultural University, Hefei, China.
J Nutr Biochem. 2021 Feb;88:108530. doi: 10.1016/j.jnutbio.2020.108530. Epub 2020 Oct 17.
Senile hypertension affects the life quality of aged population. Dietary intervention plays a pivotal role in the prevention of hypertension. There are few reports concerning the effects and mechanisms of green tea supplementation preventing age related hypertension. The current study investigated the effect and mechanism of dietary supplement of Huangshan Maofeng green tea (HSMF) on prevention of hypertension induced by deoxycorticosterone acetate (DOCA) and salt in old C57BL/6 mice. Our results showed that HSMF dose-dependently prevented the increase of systolic blood pressure and diastolic blood pressure induced by DOCA plus salt (DS) at 51-week-old mice. And HSMF significantly reduced the agonists' stimulated contraction of mesenteric arteries isolated from the old mice. The expression of vasoconstrictor genes and inflammatory cytokines in aorta were suppressed observably by HSMF supplementation compared with DS group. The protein expression of PKCα in the aorta was dose-dependently decreased by HSMF compared to DS group. The phosphorylation level of MYPT1, CPI-17and MLC was also restrained by HSMF in the aorta. Furthermore, HSMF protected kidney by maintaining integrity of glomeruli and tubules and remarkably decreased the NGAL level in plasma. HSMF also suppressed the kidney inflammation by decreasing inflammatory cytokines expression and the macrophage infiltration. Our results proved that dietary supplement of HSMF remarkably improved the vascular functions and protected kidney injury, and thus prevented hypertension induced by DS in older C57BL/6 mice. Our data indicated that the dietary supplement of HSMF may potentially be used as a food additive for preventing hypertension for aged people.
老年高血压影响老年人群的生活质量。饮食干预在预防高血压方面起着关键作用。关于补充绿茶预防与年龄相关的高血压的效果和机制的报道较少。本研究探讨了黄山毛峰绿茶（HSMF）膳食补充剂对去氧皮质酮醋酸盐（DOCA）和盐诱导的老年 C57BL/6 小鼠高血压的预防作用及其机制。我们的结果表明，HSMF 可剂量依赖性地预防 51 周龄小鼠 DOCA 加盐（DS）引起的收缩压和舒张压升高。并且 HSMF 显著降低了从老年小鼠分离的肠系膜动脉中激动剂刺激的收缩。与 DS 组相比，HSMF 补充剂可明显抑制主动脉中血管收缩基因和炎性细胞因子的表达。与 DS 组相比，HSMF 可使主动脉中 PKCα 的蛋白表达呈剂量依赖性降低。HSMF 还可抑制主动脉中 MYPT1、CPI-17 和 MLC 的磷酸化水平。此外，HSMF 通过维持肾小球和肾小管的完整性，并显著降低血浆中 NGAL 水平来保护肾脏。HSMF 还通过降低炎性细胞因子表达和巨噬细胞浸润来抑制肾脏炎症。我们的研究结果证明，HSMF 的膳食补充可显著改善血管功能，保护肾脏损伤，从而预防老年 C57BL/6 小鼠的 DS 诱导的高血压。我们的数据表明，HSMF 的膳食补充可能潜在地用作预防老年人高血压的食品添加剂。
