Machado Portilho Moyra, de Moraes Laise, Kikuti Mariana, Jacob Nascimento Leile Camila, Galvão Reis Mitermayer, Sampaio Boaventura Viviane, Khouri Ricardo, Sousa Ribeiro Guilherme
Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, Brazil.
Faculdade de Medicina, Universidade Federal da Bahia, Salvador, BA 40110-100, Brazil.
Diagnostics (Basel). 2020 Oct 18;10(10):835. doi: 10.3390/diagnostics10100835.
Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0-4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5-9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12-102, 258-260, and 722-727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.
由于寨卡病毒（ZIKV）感染与登革热病毒（DENV）存在抗原交叉反应，因此寨卡病毒感染的血清学诊断具有挑战性。本研究评估了寨卡IgM抗体捕获酶联免疫吸附测定法（美国疾病控制与预防中心寨卡IgM MAC-ELISA）在区分寨卡病毒和登革热病毒感染方面的准确性。为了确定敏感性，我们使用了21例经逆转录聚合酶链反应（RT-PCR）确诊为寨卡病毒感染患者的急性期和恢复期血清。为了确定特异性，我们使用了60例经RT-PCR确诊的登革热病例的急性期和恢复期血清以及23名献血者的血清。在疾病急性期，对于症状出现后0-4天（DPSO）采集的样本，该测定法的敏感性为12.5%（2/16），对于症状出现后5-9天采集的样本，敏感性为75.0%（3/4）。在疾病恢复期，对于症状出现后12-102天、258-260天和722-727天采集的样本，检测敏感性分别为90.9%（10/11）、100%（2/2）和0%（0/2）。RT-PCR确诊的登革热病例急性期和恢复期样本的特异性分别为100%和93.2%。献血者样本的特异性为100%。该测定法是寨卡病毒血清学诊断的准确方法，在寨卡病毒和登革热病毒共同流行的地区，已证明在监测和疫情调查中使用是可靠的。
