Graduate Program in Genetics and Molecular Biology, Laboratory of Human and Medical Genetics, Federal University of Pará, Belém, Pará 66075-110, Brazil.
Graduate Program in Oncology and Medical Sciences, Center of Oncology Research, Federal University of Pará, Belém, Pará 66063-023, Brazil.
Int J Mol Sci. 2020 Oct 16;21(20):7656. doi: 10.3390/ijms21207656.
Gastric cancer (GC) represents a notable amount of morbidity and mortality worldwide. Understanding the molecular basis of CG will offer insight into its pathogenesis in an attempt to identify new molecular biomarkers to early diagnose this disease. Therefore, studies involving small non-coding RNAs have been widely explored. Among these, PIWI-interacting RNAs (piRNAs) are an emergent class that can play important roles in carcinogenesis. In this study, small-RNA sequencing was used to identify the global piRNAs expression profile (piRNome) of gastric cancer patients. We found 698 piRNAs in gastric tissues, 14 of which were differentially expressed (DE) between gastric cancer (GC), adjacent to gastric cancer (ADJ), and non-cancer tissues (NC). Moreover, three of these DE piRNAs (piR-48966*, piR-49145, piR-31335*) were differently expressed in both GC and ADJ samples in comparison to NC samples, indicating that the tumor-adjacent tissue was molecularly altered and should not be considered as a normal control. These three piRNAs are potential risk biomarkers for GC, especially piR-48966* and piR-31335*. Furthermore, an in-silico search for mRNAs targeted by the differentially expressed piRNAs revealed that these piRNAs may regulate genes that participate in cancer-related pathways, suggesting that these small non-coding RNAs may be directly and indirectly involved in gastric carcinogenesis.
胃癌（GC）在全球范围内造成了大量的发病率和死亡率。了解 CG 的分子基础将深入了解其发病机制，试图确定新的分子生物标志物来早期诊断这种疾病。因此，涉及小非编码 RNA 的研究已经得到了广泛的探索。在这些研究中，PIWI 相互作用 RNA（piRNAs）是一类新兴的可以在致癌作用中发挥重要作用的 RNA。在这项研究中，使用小 RNA 测序来鉴定胃癌患者的全球 piRNA 表达谱（piRNome）。我们在胃组织中发现了 698 个 piRNAs，其中 14 个在胃癌（GC）、胃癌旁（ADJ）和非癌组织（NC）之间表达差异。此外，这三个差异表达的 piRNAs（piR-48966*、piR-49145 和 piR-31335*）在 GC 和 ADJ 样本中与 NC 样本相比表达不同，表明肿瘤旁组织在分子上发生了改变，不应该被视为正常对照。这三个 piRNAs 是 GC 的潜在风险生物标志物，特别是 piR-48966和 piR-31335。此外，对差异表达 piRNAs 靶向的 mRNAs 的计算搜索表明，这些 piRNAs 可能调节参与癌症相关途径的基因，这表明这些小非编码 RNA 可能直接和间接参与胃癌的发生。
Zotero 插件
