City Hospital, No. 40 St, Petersburg, 197706, Russia.
S. M. Kirov Military Medical Academy, St. Petersburg, 194044, Russia.
BMC Med Genomics. 2024 Aug 14;17(1):212. doi: 10.1186/s12920-024-01982-9.
Non-coding RNA expression has shown to have cell type-specificity. The regulatory characteristics of these molecules are impacted by changes in their expression levels. We performed next-generation sequencing and examined small RNA-seq data obtained from 6 different types of blood cells separated by fluorescence-activated cell sorting of severe COVID-19 patients and healthy control donors. In addition to examining the behavior of piRNA in the blood cells of severe SARS-CoV-2 infected patients, our aim was to present a distinct piRNA differential expression portrait for each separate cell type. We observed that depending on the type of cell, different sorted control cells (erythrocytes, monocytes, lymphocytes, eosinophils, basophils, and neutrophils) have altering piRNA expression patterns. After analyzing the expression of piRNAs in each set of sorted cells from patients with severe COVID-19, we observed 3 significantly elevated piRNAs - piR-33,123, piR-34,765, piR-43,768 and 9 downregulated piRNAs in erythrocytes. In lymphocytes, all 19 piRNAs were upregulated. Monocytes were presented with a larger amount of statistically significant piRNA, 5 upregulated (piR-49039 piR-31623, piR-37213, piR-44721, piR-44720) and 35 downregulated. It has been previously shown that piR-31,623 has been associated with respiratory syncytial virus infection, and taking in account the major role of piRNA in transposon silencing, we presume that the differential expression patterns which we observed could be a signal of indirect antiviral activity or a specific antiviral cell state. Additionally, in lymphocytes, all 19 piRNAs were upregulated.
非编码 RNA 的表达表现出细胞类型特异性。这些分子的调节特征受到其表达水平变化的影响。我们进行了下一代测序，并检查了通过严重 COVID-19 患者和健康对照供体的荧光激活细胞分选分离的 6 种不同类型的血细胞获得的小 RNA-seq 数据。除了检查严重 SARS-CoV-2 感染患者血细胞中 piRNA 的行为外，我们的目的是为每种单独的细胞类型呈现独特的 piRNA 差异表达特征。我们观察到，根据细胞类型的不同，不同分选的对照细胞（红细胞、单核细胞、淋巴细胞、嗜酸性粒细胞、嗜碱性粒细胞和中性粒细胞）具有不同的 piRNA 表达模式。在分析了严重 COVID-19 患者每一组分选细胞的 piRNA 表达后，我们观察到在红细胞中，有 3 个显著上调的 piRNA - piR-33,123、piR-34,765 和 piR-43,768，以及 9 个下调的 piRNA。在淋巴细胞中，所有 19 个 piRNA 均上调。单核细胞呈现出更多具有统计学意义的上调 piRNA，5 个上调（piR-49039、piR-31623、piR-37213、piR-44721 和 piR-44720）和 35 个下调。先前已经表明 piR-31,623 与呼吸道合胞病毒感染有关，并且考虑到 piRNA 在转座子沉默中的主要作用，我们推测我们观察到的差异表达模式可能是间接抗病毒活性的信号或特定的抗病毒细胞状态。此外，在淋巴细胞中，所有 19 个 piRNA 均上调。
