Binetti Jessica, Bertran Laia, Riesco David, Aguilar Carmen, Martínez Salomé, Sabench Fàtima, Porras Jose Antonio, Camaron Javier, Castillo Daniel Del, Richart Cristóbal, Auguet Teresa
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain.
Servei Medicina Interna, Hospital Universitari de Tarragona Joan XXIII, 43007 Tarragona, Spain.
Life (Basel). 2020 Oct 16;10(10):245. doi: 10.3390/life10100245.
Non-alcoholic fatty liver disease (NAFLD) extends from simple steatosis (SS) to non-alcoholic steatohepatitis (NASH). Peripheral serotonin (5-HT) has become as an important regulator of different metabolic pathways. 5-HT has been related to obesity and lipid accumulation in the liver. The objective of this study was to assess the relationship between the 5-HT signaling pathway and the degree of NAFLD, as well as to investigate whether peripheral 5-HT levels are related to the hepatic and jejunal mRNA abundance of serotonin receptors (HTR) in a cohort of women with morbid obesity (MO) and NAFLD. ELISA was used to quantify the serum 5-HT from normal-weight subjects (n = 26) and patients with MO (n = 58). We used RTq-PCR analysis to evaluate the relative expression of HTR in women with MO with normal liver (n = 22), SS (n = 21), and NASH (n = 15). The 5-HT was diminished in women with MO under a hypocaloric diet, regardless of the presence of NAFLD. Additionally, we report a negative correlation of 5-HT levels with metabolic syndrome criteria, suggesting that serotonin may have a protective role in obesity. Additionally, the hepatic expression of HTR2A and HTR2B were decreased in women with MO and NAFLD, but no significant differences in the HTR jejunal expression according to the presence of NAFLD were found.
非酒精性脂肪性肝病（NAFLD）范围从单纯性脂肪肝（SS）到非酒精性脂肪性肝炎（NASH）。外周血清素（5-HT）已成为不同代谢途径的重要调节因子。5-HT与肥胖及肝脏脂质蓄积有关。本研究的目的是评估5-HT信号通路与NAFLD程度之间的关系，以及调查在一组病态肥胖（MO）和NAFLD女性中，外周5-HT水平是否与血清素受体（HTR）的肝脏和空肠mRNA丰度相关。采用酶联免疫吸附测定（ELISA）法对正常体重受试者（n = 26）和MO患者（n = 58）的血清5-HT进行定量。我们使用逆转录定量聚合酶链反应（RTq-PCR）分析来评估肝脏正常（n = 22）、患SS（n = 21）和NASH（n = 15）的MO女性中HTR的相对表达。无论是否存在NAFLD，处于低热量饮食的MO女性体内5-HT水平均降低。此外，我们报告5-HT水平与代谢综合征标准呈负相关，提示血清素可能在肥胖中起保护作用。此外，MO和NAFLD女性的肝脏中HTR2A和HTR2B的表达降低，但未发现根据NAFLD的存在情况，空肠中HTR表达有显著差异。
