He Zhi-Yong, Lou Kai-Han, Zhao Jia-Hui, Zhang Ming, Zhang Lan-Chun, Li Ju, Yu Hao-Fei, Zhang Rong-Ping, Wei-Yan Hu
The Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming 650500, China.
Monash Immune Regeneration and Neuroscience Laboratories, Department of Anatomy and Developmental Biology, Monash University, Clayton, Melbourne 3800, Australia.
Evid Based Complement Alternat Med. 2020 Oct 7;2020:2305784. doi: 10.1155/2020/2305784. eCollection 2020.
To investigate the protective effects and possible mechanisms of action of resina draconis (RD) on acute liver injury and liver regeneration after 2/3 partial hepatectomy (PH) in mice.
2/3 PH was used to induce acute liver injury. Mice were divided into three groups: sham, vehicle + 2/3 PH, and RD + 2/3 PH. Resina draconis was administered intragastrically after 2/3 PH into the RD + 2/3 PH group, and the same volume of vehicle (1% sodium carboxymethyl cellulose) was injected into the vehicle + 2/3 PH group and sham group mice. The index of liver to body weight (ILBW) and proliferating cell nuclear antigen (PCNA) were assayed to evaluate liver regeneration. Blood and liver tissues were collected for serological and western blotting analysis.
Resina draconis protected against 2/3 PH-induced acute severe liver injury and promoted liver regeneration as shown by significantly increased ILBW compared with that of controls. 2/3 PH increased serum AST and ALT levels, which were significantly decreased by RD treatment, while 2/3 PH decreased serum TP and ALB, which were increased by RD treatment. In the RD + 2/3 PH group, PCNA expression was significantly increased compared with the 2/3 PH group. Further, hepatocyte growth factor (HGF), TNF, and EGFR levels were increased in the RD group at postoperative days 2 and 4 compared with the those in the 2/3 PH group.
Our results suggest that RD ameliorates acute hepatic injury and promotes liver cell proliferation, liver weight restoration, and liver function after 2/3 PH, probably via HGF, TNF, and EGFR signaling.
探讨血竭对小鼠2/3部分肝切除（PH）后急性肝损伤及肝再生的保护作用及其可能的作用机制。
采用2/3 PH诱导急性肝损伤。将小鼠分为三组：假手术组、溶剂+2/3 PH组和血竭+2/3 PH组。2/3 PH术后对血竭+2/3 PH组小鼠进行灌胃给予血竭，对溶剂+2/3 PH组和假手术组小鼠注射相同体积的溶剂（1%羧甲基纤维素钠）。检测肝重与体重比值（ILBW）和增殖细胞核抗原（PCNA）以评估肝再生情况。采集血液和肝组织进行血清学和蛋白质印迹分析。
血竭可预防2/3 PH诱导的急性严重肝损伤，并促进肝再生，与对照组相比，ILBW显著增加。2/3 PH可使血清AST和ALT水平升高，血竭治疗可使其显著降低，而2/3 PH可使血清TP和ALB降低，血竭治疗可使其升高。与2/3 PH组相比，血竭+2/3 PH组PCNA表达显著增加。此外，与2/3 PH组相比，血竭组术后第2天和第4天肝细胞生长因子（HGF）、肿瘤坏死因子（TNF）和表皮生长因子受体（EGFR）水平升高。
我们的结果表明，血竭可改善2/3 PH后的急性肝损伤，促进肝细胞增殖、肝重恢复和肝功能，可能是通过HGF、TNF和EGFR信号通路实现的。
