miR-215对视网膜母细胞瘤细胞系中肿瘤抑制基因Rb1表达的影响
Effect of miR-215 on the Expression of Tumor Suppressor Gene Rb1 in Retinoblastoma Cell Lines.
作者信息
Shao Liqin, Sheng Zhangxing, Zhu Yuefeng, Li Jianchao, Meng Rufa
机构信息
Department of Ophthalmology, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang 312000, China.
Department of Ophthalmology, Xi'an Traditional Chinese Medicine Hospital, Xi'an, Shaanxi 710020, China.
出版信息
Iran J Public Health. 2020 Jul;49(7):1298-1306. doi: 10.18502/ijph.v49i7.3583.
BACKGROUND
Effect of miR-215 on the expression of tumor suppressor gene retinoblastoma (Rb)1 in Rb cell lines was investigated.
METHODS
A total of 128 patients were selected. The expression of miR-215 in cancer and adjacent healthy tissues of the 128 patients was detected by reverse transcription-quantitative PCR (RT-qPCR). HXO-Rb44 and Y79 cell lines were transfected with miR-215 analogs or miR-215 inhibitors, and the expression of Rb1 protein in the cell lines was detected by western blotting.
RESULTS
The expression of miR-215 in the adjacent healthy tissues of patients was significantly lower than that in cancer tissues (0.001). The expression of miR-215 in Y79 and HXO-Rb44 cells was significantly higher than that in APRE-19 cells (0.001). The expression of miR-215 in HXO-Rb44 cells was significantly higher than that in Y79 cells (0.001). The expression of miR-215 was statistically different from the degree of differentiation and nerve infiltration (0.05). The expression of Rb1 in cancer tissues was significantly lower than that in adjacent tissues (0.001), the expression of APRE-19 was significantly higher than that in Y79 and HXO-Rb44 cells (0.001), and the expression of Rb1 in HXO-Rb44 cells was significantly higher than that in Y79 cells (0.05). There was a negative correlation between miR-215 and Rb1 in the tissues of patients, and Rb1 expression decreased with the increase of miR-215 (r=-0.576, 0.001).
CONCLUSION
miR-215 is highly expressed in Rb cell lines, and is related to the clinicopathological features of this disease.
背景
研究了miR-215对视网膜母细胞瘤（Rb）细胞系中肿瘤抑制基因Rb1表达的影响。
方法
共选取128例患者。采用逆转录定量聚合酶链反应（RT-qPCR）检测128例患者癌组织及癌旁健康组织中miR-215的表达。将miR-215类似物或miR-215抑制剂转染至HXO-Rb44和Y79细胞系，采用蛋白质免疫印迹法检测细胞系中Rb1蛋白的表达。
结果
患者癌旁健康组织中miR-215的表达明显低于癌组织（P<0.001）。Y79和HXO-Rb44细胞中miR-215的表达明显高于APRE-19细胞（P<0.001）。HXO-Rb44细胞中miR-215的表达明显高于Y79细胞（P<0.001）。miR-215的表达与分化程度和神经浸润存在统计学差异（P<0.05）。癌组织中Rb1的表达明显低于癌旁组织（P<0.001），APRE-19的表达明显高于Y79和HXO-Rb44细胞（P<0.001），HXO-Rb44细胞中Rb1的表达明显高于Y79细胞（P<0.05）。患者组织中miR-215与Rb1呈负相关，Rb1表达随miR-215升高而降低（r=-0.576，P<0.001）。
结论
miR-215在Rb细胞系中高表达，且与该疾病的临床病理特征相关。
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