Ghatreh Samani Keihan, Farrokhi Effat, Tabatabaee Aliye, Jalilian Narges, Jafari Mahbube
Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Iran J Public Health. 2020 Jul;49(7):1324-1329. doi: 10.18502/ijph.v49i7.3586.
Tamoxifen (TAM) is widely used for adjuvant therapy in breast cancer patients. Tamoxifen therapy may lead to serious side effects. Anti-apoptotic substances in combination with chemotherapy drugs can result in additive or synergistic effects. Lauryl gallate (LG), a Gallic acid derivative, has been proven to inhibit tumor growth, without affecting normal cells. This study aimed to investigate the synergistic effect of TAM and LG in breast cancer cell line (MCF-7).
In this experimental study, performed in ShahreKord University of Medical Science, Iran in 2017, the MCF-7 cells were treated by final concentrations of 10 μM TAM alone, and in combination with 200 μM of LG. We also used EX-527, as SIRT-1 inhibitor to examine the role of SIRT1 in cell apoptosis. and gene expression were measured by real-time PCR method, and cell apoptosis was investigated by flow cytometry.
Tamoxifen alone and in combination with LG decreased expression 2.64±0.75 and 6.38±1.9 fold, respectively, after 48 h (<0.05). expression was increased 1.67±0.22 and 2.47±0.34 - fold by TAM alone and in combination with LG, respectively (<0.05). TAM alone and in combination with LG increased the percentage of apoptotic cells 15.79±2.81 and 60.67±6.23 percent, respectively after 48 h (<0.001).
The combination of LG and TAM is more effective for induction of apoptosis of breast cancer cells, compared to individual use of each. Thus, our data pave the way for new therapeutic options for suppressing breast cancer growth.
他莫昔芬（TAM）广泛用于乳腺癌患者的辅助治疗。他莫昔芬治疗可能会导致严重的副作用。抗凋亡物质与化疗药物联合使用可产生相加或协同作用。没食子酸月桂酯（LG）是一种没食子酸衍生物，已被证明可抑制肿瘤生长，且不影响正常细胞。本研究旨在探讨TAM与LG在乳腺癌细胞系（MCF-7）中的协同作用。
在2017年于伊朗设拉子医科大学进行的这项实验研究中，MCF-7细胞分别用终浓度为10 μM的TAM单独处理以及与200 μM的LG联合处理。我们还使用EX-527作为SIRT-1抑制剂来研究SIRT1在细胞凋亡中的作用。通过实时PCR法检测 和 基因表达，并通过流式细胞术研究细胞凋亡。
48小时后，单独使用他莫昔芬以及与LG联合使用分别使 表达降低了2.64±0.75倍和6.38±1.9倍（<0.05）。单独使用TAM以及与LG联合使用分别使 表达增加了1.67±0.22倍和2.47±0.34倍（<0.05）。48小时后，单独使用他莫昔芬以及与LG联合使用分别使凋亡细胞百分比增加了15.79±2.81%和60.67±6.23%（<0.001）。
与单独使用每种药物相比，LG与TAM联合使用对诱导乳腺癌细胞凋亡更有效。因此，我们的数据为抑制乳腺癌生长的新治疗选择铺平了道路。
