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表没食子儿茶素-3-没食子酸酯（EGCG）、三氧化二砷与放疗联合治疗对乳腺癌细胞系的协同作用评估

Assessment Synergistic Effects of Integrated Therapy with Epigallocatechin-3-Gallate (EGCG) & Arsenic Trioxide and Irradiation on Breast Cancer Cell Line.

作者信息

Changizi Vahid, Azariasl Samayeh, Motevaseli Elahe, Jafari Nodooshan Saeedeh

机构信息

Department of Technology of Radiology and Radiotherapy, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.

Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Public Health. 2020 Aug;49(8):1555-1563. doi: 10.18502/ijph.v49i8.3901.

DOI:10.18502/ijph.v49i8.3901
PMID:33083333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7554386/
Abstract

BACKGROUND

Breast cancer is the most common invasive malignancy among women in the world. The current breast cancer therapies pose significant clinical challenges. Low-dose chemotherapy represents a new strategy to treat solid tumors in combination with natural products such as green tea catechins. Epigallocatechin-3-gallate (EGCG) is the major polyphenolic extract from green tea with potent anticancer and antioxidant effects. The purpose of this study was to investigate the effects of EGCG, Arsenic trioxide (ATO) and gamma radiation on MCF-7 cell line.

METHODS

The anti-proliferative effects of EGCG and ATO individually, moreover in combination with radiation on MCF-7 cells were evaluated with MTT assay. The expression of apoptotic gens (Bax, Bcl-2, Caspase-3 and Fas) was assessed by real-time PCR.

RESULTS

Based on the results of MTT assay, EGCG and ATO exhibited dose and time-dependent anti-proliferative effects on MCF-7 cells. The combined therapy of EGCG and ATO in presence and absence radiation could rise cell death up to 80%. Moreover, integrated therapy made Bax up-regulated and Bcl-2 down- regulated.

CONCLUSION

In assessment synergistic effects of integrated therapy with EGCG and ATO and irradiation had been significant impact on low dose chemotherapy for breast cancer treatment.

摘要

背景

乳腺癌是全球女性中最常见的侵袭性恶性肿瘤。目前的乳腺癌治疗方法面临重大临床挑战。低剂量化疗是一种结合绿茶儿茶素等天然产物治疗实体瘤的新策略。表没食子儿茶素-3-没食子酸酯（EGCG）是绿茶中的主要多酚提取物，具有强大的抗癌和抗氧化作用。本研究的目的是探讨EGCG、三氧化二砷（ATO）和γ射线对MCF-7细胞系的影响。

方法

采用MTT法评估EGCG和ATO单独以及与辐射联合对MCF-7细胞的抗增殖作用。通过实时PCR评估凋亡基因（Bax、Bcl-2、Caspase-3和Fas）的表达。

结果

基于MTT法结果，EGCG和ATO对MCF-7细胞表现出剂量和时间依赖性的抗增殖作用。EGCG和ATO在有或无辐射情况下的联合治疗可使细胞死亡高达80%。此外，联合治疗使Bax上调，Bcl-2下调。

结论

评估EGCG和ATO联合放疗的协同作用对乳腺癌低剂量化疗有显著影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/6d247e1089da/IJPH-49-1555-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/d6ae74373469/IJPH-49-1555-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/0d03502de6f2/IJPH-49-1555-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/4e2d05e6f71b/IJPH-49-1555-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/17388646d76b/IJPH-49-1555-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/ae6f85ed5247/IJPH-49-1555-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/6d247e1089da/IJPH-49-1555-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/d6ae74373469/IJPH-49-1555-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/0d03502de6f2/IJPH-49-1555-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/4e2d05e6f71b/IJPH-49-1555-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/17388646d76b/IJPH-49-1555-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/ae6f85ed5247/IJPH-49-1555-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eeb/7554386/6d247e1089da/IJPH-49-1555-g006.jpg

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