Davhana N C, ElBakri A K, Bessong P O, Samie A
Molecular Parasitology and Opportunistic Infection Program, Department of Microbiology, University of Venda, Private Bag X5050, Thohoyandou, 0950, South Africa.
Department of Medical Laboratory Sciences, College of Health Sciences, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates.
Heliyon. 2020 Oct 7;6(10):e05129. doi: 10.1016/j.heliyon.2020.e05129. eCollection 2020 Oct.
Intestinal parasitic diseases are common in developing countries including South Africa and have been documented to be the most common in children under the age of five. The present study aimed to identify any potential association that may exist between TNF-α promoter gene polymorphism and parasitic infections. A total of 199 blood samples were evaluated from children who were part of the MAL-ED study cohort. The DNA was used to investigate polymorphism in the promoter region of the TNF-α gene at position -1031T/C. The polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The TC genotype at position -1031 was significantly higher in healthy controls children than in children who were infected with (59.9% vs 29.4%, P = 0.015) and (59.1% vs 30.8%, P = 0.046), indicating that TC genotype may be protective against infections and infections. The CC genotype at position -1031 was more common among children with parasite and diarrhea and the results was statistically significant (P = 0.04). This study has revealed that the CC genotype may be is a risk factor for symptomatic parasitic infections while the TC genotype might be protective of infections among children in Dzimauli community.
肠道寄生虫病在包括南非在内的发展中国家很常见，并且有记录表明在五岁以下儿童中最为普遍。本研究旨在确定肿瘤坏死因子-α（TNF-α）启动子基因多态性与寄生虫感染之间可能存在的任何潜在关联。对来自MAL-ED研究队列的儿童的总共199份血样进行了评估。使用DNA来研究TNF-α基因启动子区域在-1031T/C位置的多态性。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析检测多态性。-1031位置的TC基因型在健康对照儿童中显著高于感染[未提及具体寄生虫名称1]的儿童（59.9%对29.4%，P = 0.015）以及感染[未提及具体寄生虫名称2]的儿童（59.1%对30.8%，P = 0.046），表明TC基因型可能对[未提及具体寄生虫名称1]感染和[未提及具体寄生虫名称2]感染具有保护作用。-1031位置的CC基因型在患有寄生虫和腹泻的儿童中更为常见，结果具有统计学意义（P = 0.04）。本研究表明，在齐马乌利社区儿童中，CC基因型可能是有症状寄生虫感染的危险因素，而TC基因型可能对[未提及具体寄生虫名称1]感染具有保护作用。
