Department of Internal Medicine, Division of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, PR China.
Department of Physiology & Pathophysiology, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, PR China.
Epigenomics. 2020 Dec;12(24):2189-2204. doi: 10.2217/epi-2020-0155. Epub 2020 Oct 21.
To explore the potentially important role of miRNA 146b-5p (miR-146b) during the development of atherosclerosis. Proliferation, migration and luciferase assays and mouse models were used to determine the functions of miR-146b. miR-146b was identified as substantially upregulated in the aortic plaques of -/- mice as well as in response to inflammatory cytokines. Overexpression of miR-146b repressed proliferation and migration of vascular smooth muscle cells by downregulating and , respectively. Adeno-associated virus-mediated miR-146b overexpression inhibited neointima formation after carotid injury and suppressed atherosclerotic plaque formation in Western diet-induced -/- mice. miR-146b is a novel regulator of vascular smooth muscle cell function induced by inflammatory response, specifically in neointima formation, and offers a novel therapeutic strategy for treating atherosclerosis.
探讨 microRNA 146b-5p（miR-146b）在动脉粥样硬化发展过程中的潜在重要作用。采用增殖、迁移和荧光素酶检测以及小鼠模型来确定 miR-146b 的功能。miR-146b 在-/-小鼠的主动脉斑块中以及对炎性细胞因子的反应中均显著上调。miR-146b 的过表达通过下调和分别抑制血管平滑肌细胞的增殖和迁移。腺相关病毒介导的 miR-146b 过表达抑制颈动脉损伤后的新生内膜形成，并抑制 Western 饮食诱导的-/-小鼠的动脉粥样硬化斑块形成。miR-146b 是炎症反应诱导的血管平滑肌细胞功能的新型调节因子，特别是在新生内膜形成中，为治疗动脉粥样硬化提供了一种新的治疗策略。
