Department of Neurology, The First Affiliated Hospital of Shandong First Medical University, No. 16766 Jingshi Road, Lixia District, Jinan, 250014, Shandong, People's Republic of China.
Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, No. 16766 Jingshi Road, Lixia District, Jinan, 250014, Shandong, People's Republic of China.
Neuromolecular Med. 2020 Dec;22(4):493-502. doi: 10.1007/s12017-020-08606-2. Epub 2020 Oct 21.
Galuteolin, a Chinese herbal medicine, purified from Lonicera Japonica. In this study, we aimed to investigate the neuroprotective effect of galuteolin against cerebral ischemia/reperfusion (I/R) injury. We administered galuteolin or galuteolin and rapamycin to rats which had middle cerebral artery occlusion/reperfusion (MCAO/R). A series of characterizations were carried out to monitor the outcomes of galuteolin in I/R rats regarding the infarct volumes, neurological deficits, and brain water, as well as its effect on neuroprotection and autophagy. It was found that galuteolin significantly reduced the infarct volume, brain water content, and the neurological deficits in a dose-dependent manner. Neuron damages were decreased in the hippocampal carotid artery 1 pyramidal layer by galuteolin. The expression levels of neuron-specific enolase (NSE) increased after galuteolin treatment. Galuteolin significantly decreased the expression levels of autophagy-related proteins. In addition, galuteolin decreased rapamycin-related neuron damages and activations of autophagy in I/R rats. Our data suggested that galuteolin can inhibit ischemic brain injuries through the regulation of autophagy-related indicators in I/R.
汉防己甲素，一种从忍冬科植物中提取的中药。本研究旨在探讨汉防己甲素对脑缺血再灌注（I/R）损伤的神经保护作用。我们给大脑中动脉闭塞/再灌注（MCAO/R）的大鼠施用汉防己甲素或汉防己甲素和雷帕霉素。进行了一系列特征描述，以监测汉防己甲素在 I/R 大鼠中的梗死体积、神经功能缺损和脑水肿情况，以及其对神经保护和自噬的影响。结果发现，汉防己甲素能显著降低梗死体积、脑水肿程度和神经功能缺损，呈剂量依赖性。汉防己甲素可减少海马 CA1 锥体神经元损伤。汉防己甲素处理后神经元特异性烯醇化酶（NSE）的表达水平增加。汉防己甲素可显著降低自噬相关蛋白的表达水平。此外，汉防己甲素可减少 I/R 大鼠中雷帕霉素相关的神经元损伤和自噬的激活。我们的数据表明，汉防己甲素可通过调节 I/R 中与自噬相关的指标来抑制缺血性脑损伤。
