Department of Ophthalmology, College of Medicine, Kosin University, Gospel Hospital , Busan.
Strabismus. 2020 Dec;28(4):201-207. doi: 10.1080/09273972.2020.1832546. Epub 2020 Oct 21.
To elucidate the differences in muscle bundle and satellite cells in medial rectus muscle through histological and Immunofluorescence studies of intermittent exotropia patients and normal controls. : From January 2015 to December 2017, 15 patients who underwent medial rectus resection surgery at Kosin University Gospel Hospital were enrolled. Four medial recti muscles collected from two brain-dead men without strabismus were used as controls and compared with the intermittent exotropia group. Hematoxylin and eosin (HE) staining were performed, and all muscle bundle diameters were measured with the Image J program and compared to the mean value. Immunological staining for MyoHC (Myosin Heavy Chain), PAX7 (Transcription Factor), and PCNA (Proliferating Cell Nuclear Antigen) were performed to analyze the distribution of myocytes and PAX7-positive and PCNA-positive cells. : The mean ages of the strabismus and control groups were 17.33 ± 13.05 and 22.0 ± 5.85 years, respectively, and the male to female ratio was 7:2 and 2:0. The average angle of deviation in the exotropia patients was 36.0 ± 16.83 prism diopters. The mean muscle bundle diameter under light microscopy was 60.21 ± 1.48 in the exotropia group and 52.27 ± 0.74 in the control group. The exotropia group showed significantly greater mean muscle bundle diameter ( < .001) and diameter regularity than the control group. The PAX7(+)/muscle bundle number ratio was 0.016 ± 0.014 and 0.056 ± 0.015 in the exotropia group and control group, respectively ( < .001), and the PCNA(+)/muscular bundle number ratio was 0.015 ± 0.017 and 0.182 ± 0.102 ( < .001). Both were significantly higher in the control group compared to that in the exotropia patients. : In the exotropia group, mean diameter of medial rectus muscle bundle was significantly larger than that of control group. The ratios of PAX7 and PCNA to muscle bundle number were significantly higher in the control group than intermittent extropia group. We found the negative relationship between activation of satellite cells and muscle bundle diameter, and it might take one step forward to elucidate the pathogenesis of intermittent extropia.
通过对间歇性外斜视患者和正常对照的组织学和免疫荧光研究，阐明内直肌肌束和卫星细胞的差异。：2015 年 1 月至 2017 年 12 月，在 Kosin 大学福音医院接受内直肌切除术的 15 名患者被纳入研究。从两名无斜视的脑死亡男性中收集了 4 条内直肌作为对照，并与间歇性外斜视组进行比较。进行苏木精和伊红（HE）染色，并使用 Image J 程序测量所有肌束直径，并与平均值进行比较。进行肌球蛋白重链（MyoHC）、PAX7（转录因子）和 PCNA（增殖细胞核抗原）的免疫染色，以分析肌细胞和 PAX7 阳性和 PCNA 阳性细胞的分布。：斜视组和对照组的平均年龄分别为 17.33±13.05 岁和 22.0±5.85 岁，男女比例分别为 7:2 和 2:0。外斜视患者的平均斜视角度为 36.0±16.83 棱镜度。在斜视组中，光镜下肌束直径的平均值为 60.21±1.48，在对照组中为 52.27±0.74。斜视组的平均肌束直径明显大于对照组（<.001），且直径规律性也明显大于对照组（<.001）。PAX7（+）/肌束数比分别为斜视组和对照组的 0.016±0.014 和 0.056±0.015（<.001），PCNA（+）/肌束数比分别为斜视组和对照组的 0.015±0.017 和 0.182±0.102（<.001）。与斜视患者相比，对照组均明显更高。：在斜视组中，内直肌肌束的平均直径明显大于对照组。对照组中 PAX7 和 PCNA 与肌束数的比值明显高于间歇性外斜视组。我们发现卫星细胞激活与肌束直径之间存在负相关关系，这可能进一步阐明间歇性外斜视的发病机制。
