评价珍华酊方对人癌细胞系的细胞毒性。
Evaluation of Tegaran Formula ZhenHua cytotoxicity against human cancer cell lines.
机构信息
Research Genetic Cancer Centre S.A., Industrial Area of Florina, Florina, Greece.
Research Genetic Cancer Centre International GmbH Headquarters, Baarerstrasse, Zug, Switzerland.
出版信息
PLoS One. 2020 Oct 21;15(10):e0240969. doi: 10.1371/journal.pone.0240969. eCollection 2020.
The aim of this study is to evaluate the potential health effects of Tegaran Formula ZhenHua, a nutritional supplement used mainly by cancer patients. Its active ingredients and cytotoxicity was assessed with analytical methods and viability assays, respectively. The analytical methods consisted of dissolution, disintegration, HPLC, LC/MS, GC/MS and NMR. Cytotoxicity was assessed by MTT, SRB, CVE colorimetric viability assays in 0, 24, 48 and 72h time points. The results indicate that Tegaran Formula ZhenHua supplement did not present any cytotoxic effects due to issues related to the capsules' solubility, distribution and identification of the active ingredient.
本研究旨在评估特佳然方振华（Tegaran Formula ZhenHua）的潜在健康影响，该营养补充剂主要用于癌症患者。其活性成分和细胞毒性分别采用分析方法和活力测定法进行评估。分析方法包括溶解、崩解、HPLC、LC/MS、GC/MS 和 NMR。细胞毒性通过 MTT、SRB、CVE 比色活力测定法在 0、24、48 和 72 小时时间点进行评估。结果表明，特佳然方振华补充剂由于胶囊的溶解度、分布和活性成分的鉴定等问题，没有表现出任何细胞毒性作用。
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