Wang Jie, Xue Kaili, Yi Ping, Zhu Xiaojie, Peng Qingjie, Wang Zijian, Peng Yongchong, Chen Yingyu, Robertson Ian D, Li Xiang, Guo Aizhen, Aleri Joshua W
The State Key Laboratory of Agricultural Microbiology, Wuhan 430070, China.
School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch 6150, Western Australia, Australia.
Pathogens. 2020 Oct 19;9(10):853. doi: 10.3390/pathogens9100853.
The objective of this study was to estimate the fecal carriage of spp. among culled adult dairy cows presented to an abattoir in Wuhan, China and to evaluate their antimicrobial resistance profiles. Rectal swabs from 138 culled cows were cultured. Laboratory analysis involved the identification of , the susceptibility assessment and the presence of Extended Spectrum β-lactamases and genes in the isolates. An overall prevalence of of 29.0% was recorded with 63.4% (26/41) and 2.4% (1/41) of the isolates identified as . Typhimurium and . Dublin, respectively. The occurrence of was higher (odd ratios: 3.3) in culled cows originating from the northeast zone of China than cows originating from the central and north zones. Twenty multi-drug resistant strains (resistant to three or more antimicrobial agents) were detected (48.8%) and overall, a high resistance to ampicillin (36/41) and tetracycline (15/41) was observed. Extended Spectrum β-lactamases phenotypes were found in 7/41 isolates, of which all contained the bla resistance gene, and no genes were found by polymerase chain reaction. The high prevalence of fecal carriage and antimicrobial resistance may contribute to an increased risk of transmission to food.
本研究的目的是估计中国武汉一家屠宰场宰杀的成年奶牛中 spp. 的粪便携带情况，并评估其抗菌药物耐药谱。对138头宰杀奶牛的直肠拭子进行培养。实验室分析包括 的鉴定、药敏评估以及分离株中广谱β-内酰胺酶和 基因的检测。记录的总体携带率为29.0%，分别有63.4%（26/41）和2.4%（1/41）的分离株被鉴定为鼠伤寒沙门氏菌和都柏林沙门氏菌。来自中国东北地区的宰杀奶牛中 的发生率高于来自中部和北部地区的奶牛（优势比：3.3）。检测到20株多重耐药菌株（对三种或更多抗菌药物耐药）（48.8%），总体上，观察到对氨苄西林（36/41）和四环素（15/41）的高耐药性。在41株分离株中有7株发现了广谱β-内酰胺酶表型，其中所有菌株都含有bla耐药基因，通过聚合酶链反应未发现 基因。 粪便携带和抗菌药物耐药性的高流行率可能会增加 传播到食物中的风险。
