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猪 TRIM26 的表达谱及其对干扰素-β产生和抗病毒反应的抑制作用。

Expression Profile of Porcine TRIM26 and Its Inhibitory Effect on Interferon-β Production and Antiviral Response.

机构信息

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Genes (Basel). 2020 Oct 19;11(10):1226. doi: 10.3390/genes11101226.

DOI:10.3390/genes11101226
PMID:33086712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7589756/
Abstract

TRIM26, a member of the tripartite motif (TRIM) family has been shown to be involved in modulation of innate antiviral response. However, the functional characteristics of porcine TRIM26 (porTRIM26) are unclear. In this study, we used a synthesized antigen peptide to generate a polyclonal antibody against porTRIM26 with which to study the expression and function of porTRIM26. We demonstrated that polyinosinic:polycytidylic acid (poly (I:C)) stimulation and viral infection (vesicular stomatitis (VSV) or porcine reproductive and respiratory syndrome virus (PRRSV)) induce expression of porTRIM26, whereas knock-down expression of porTRIM26 promotes interferon (IFN)- production after poly (I:C) stimulation and virus infection (VSV or PRRSV). The importance of the porTRIM26-mediated modulation of the antiviral response was also shown in VSV- or PRRSV-infected cells. In summary, these findings show that porTRIM26 has an inhibitory role in IFN- expression and the antiviral response.

摘要

TRIM26 是三部分基序 (TRIM) 家族的成员，已被证明参与调节先天抗病毒反应。然而，猪 TRIM26（porTRIM26）的功能特征尚不清楚。在这项研究中，我们使用合成的抗原肽生成了针对 porTRIM26 的多克隆抗体，用于研究 porTRIM26 的表达和功能。我们证明了多聚肌苷酸：多聚胞苷酸（poly（I:C））刺激和病毒感染（水疱性口炎病毒（VSV）或猪繁殖与呼吸综合征病毒（PRRSV））诱导 porTRIM26 的表达，而 porTRIM26 的敲低表达促进了 poly（I:C）刺激和病毒感染（VSV 或 PRRSV）后的干扰素（IFN）-产生。在 VSV 或 PRRSV 感染的细胞中，还证明了 porTRIM26 介导的抗病毒反应调节的重要性。总之，这些发现表明 porTRIM26 在 IFN-表达和抗病毒反应中具有抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/759b9b84dbd1/genes-11-01226-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/13132c51b36b/genes-11-01226-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/e3da78b68c43/genes-11-01226-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/52b6f5e3122e/genes-11-01226-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/3d7227c2bced/genes-11-01226-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/759b9b84dbd1/genes-11-01226-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/13132c51b36b/genes-11-01226-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/e3da78b68c43/genes-11-01226-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/52b6f5e3122e/genes-11-01226-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/3d7227c2bced/genes-11-01226-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c722/7589756/759b9b84dbd1/genes-11-01226-g005.jpg

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Ubiquitin signaling in immune responses.
Front Vet Sci. 2022 Sep 26;9:1010039. doi: 10.3389/fvets.2022.1010039. eCollection 2022.
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Expression Analysis of Outer Membrane Protein HPS_06257 in Different Strains of and Its Potential Role in Protective Immune Response against HPS_06257-Expressing Strains via Antibody-Dependent Phagocytosis.不同菌株中外膜蛋白HPS_06257的表达分析及其通过抗体依赖性吞噬作用对表达HPS_06257菌株的保护性免疫反应中的潜在作用。
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Regulation of Tripartite Motif-Containing Proteins on Immune Response and Viral Evasion.含三联基序蛋白对免疫应答及病毒逃逸的调控
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