Department of Histology and Embryology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.
Department of Medical Biology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.
Nutr Cancer. 2021;73(11-12):2532-2537. doi: 10.1080/01635581.2020.1836244. Epub 2020 Oct 21.
Endometrial cancer is the most common type of cancer in the female reproductive system. Geraniol is acyclic monoterpene alcohol derived from essential oils of aromatic plants. This study aimed to investigate the apoptosis pathway of geraniol on Ishikawa cells. The cytotoxic effects of Geraniol on Ishikawa cells were determined by an MTT test. Ishikawa cells were seeded on cover slips, the IC50 dose was applied, and the cells were incubated with antibodies against Bax, Bcl-2, and TUNEL Assay. mRNA expression analysis of apoptosis-related genes was determined by RT-qPCR with an IC50 dose of Geraniol. The IC50 dose of Geraniol decreased Bcl-2 staining significantly, but it significantly increased Bax staining and TUNEL positive cells. A significant increase in the Bax, caspase3, caspase-8, cytochrome C and Fas genes and a significant decrease in the Bcl-2 gene was observed when the IC50 dose group was compared to the cells in the control group based on their mRNA expression levels.Analysis of expression of genes whose products are involved in apoptosis suggests the involvement of the mitochondrial pathway.
子宫内膜癌是女性生殖系统最常见的癌症类型。香叶醇是一种从芳香植物精油中提取的无环单萜醇。本研究旨在探讨香叶醇对 Ishikawa 细胞凋亡途径的影响。通过 MTT 试验测定香叶醇对 Ishikawa 细胞的细胞毒性作用。将 Ishikawa 细胞接种在盖玻片上，应用 IC50 剂量，并孵育抗 Bax、Bcl-2 和 TUNEL 检测抗体。用 RT-qPCR 测定凋亡相关基因的 mRNA 表达分析，用香叶醇的 IC50 剂量处理。IC50 剂量的香叶醇显著降低了 Bcl-2 的染色，但显著增加了 Bax 的染色和 TUNEL 阳性细胞。与对照组细胞相比，IC50 剂量组 Bax、caspase3、caspase-8、细胞色素 C 和 Fas 基因显著增加，Bcl-2 基因显著减少。基于其 mRNA 表达水平，分析参与凋亡的基因产物的表达表明线粒体途径的参与。
