神秘而迷人：DNA 聚合酶 ɩ 在发现 20 年后仍然扑朔迷离。
Mysterious and fascinating: DNA polymerase ɩ remains enigmatic 20 years after its discovery.
机构信息
Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-3371, USA.
Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-3371, USA.
出版信息
DNA Repair (Amst). 2020 Sep;93:102914. doi: 10.1016/j.dnarep.2020.102914.
Abstract
With the publication of the first paper describing the biochemical properties of DNA polymerase iota (polɩ), the question immediately arose as to why cells harbor such a low-fidelity enzyme which often violates the Watson-Crick base pairing rules? Yet 20 years after its discovery, the cellular function of polɩ remains unknown. Here, we provide a graphical review of the unique biochemical properties of polɩ and speculate about the cellular pathways in which enigmatic polɩ may participate.
摘要
随着第一篇描述 DNA 聚合酶iota（polɩ）生化特性的论文发表，人们立即提出了这样一个问题：为什么细胞中存在这种低保真度的酶，它经常违反沃森-克里克碱基配对规则？然而，在它被发现 20 年后，polɩ 的细胞功能仍然未知。在这里，我们提供了一个关于 polɩ 独特生化特性的图形化综述，并推测了神秘的 polɩ 可能参与的细胞途径。
相似文献
1
Mysterious and fascinating: DNA polymerase ɩ remains enigmatic 20 years after its discovery.神秘而迷人：DNA 聚合酶 ɩ 在发现 20 年后仍然扑朔迷离。
DNA Repair (Amst). 2020 Sep;93:102914. doi: 10.1016/j.dnarep.2020.102914.
2
Preferential incorporation of G opposite template T by the low-fidelity human DNA polymerase iota.低保真度的人类DNA聚合酶ι使鸟嘌呤优先掺入与模板胸腺嘧啶相对应的位置。
Mol Cell Biol. 2000 Oct;20(19):7099-108. doi: 10.1128/MCB.20.19.7099-7108.2000.
3
Polymerase iota - an odd sibling among Y family polymerases.聚合酶iota——Y 家族聚合酶中的一个奇异兄弟。
DNA Repair (Amst). 2020 Feb;86:102753. doi: 10.1016/j.dnarep.2019.102753. Epub 2019 Nov 20.
4
The steric gate of DNA polymerase ι regulates ribonucleotide incorporation and deoxyribonucleotide fidelity.DNA 聚合酶 ι 的立体位阻门控调节核苷酸的掺入和脱氧核苷酸的保真度。
J Biol Chem. 2014 Mar 28;289(13):9136-45. doi: 10.1074/jbc.M113.545442. Epub 2014 Feb 14.
5
Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue HO Sensitivity in -Deficient Cells.三种具有聚合酶活性缺陷的人 Pol ι 变体未能挽救 - 缺陷细胞的 HO 敏感性。
Chem Res Toxicol. 2020 Aug 17;33(8):2120-2129. doi: 10.1021/acs.chemrestox.0c00127. Epub 2020 Jul 23.
6
Biochemical evidence for the requirement of Hoogsteen base pairing for replication by human DNA polymerase iota.关于人类DNA聚合酶ι进行复制时对Hoogsteen碱基配对需求的生化证据。
Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10466-71. doi: 10.1073/pnas.0503859102. Epub 2005 Jul 13.
7
DNA polymerases: Hoogsteen base-pairing in DNA replication?DNA聚合酶：DNA复制中的Hoogsteen碱基配对？
Nature. 2005 Sep 15;437(7057):E6-7; discussion E7. doi: 10.1038/nature04199.
8
DNA polymerase ι: The long and the short of it!DNA聚合酶ι：其长短之述！
DNA Repair (Amst). 2017 Oct;58:47-51. doi: 10.1016/j.dnarep.2017.08.006. Epub 2017 Aug 19.
9
Structure of human DNA polymerase iota and the mechanism of DNA synthesis.人源 DNA 聚合酶 ι的结构与 DNA 合成机制
Biochemistry (Mosc). 2012 Jun;77(6):547-61. doi: 10.1134/S0006297912060016.
10
Biochemical characterization of human DNA polymerase iota provides clues to its biological function.人类DNA聚合酶ι的生化特性为其生物学功能提供了线索。
Biochem Soc Trans. 2001 May;29(Pt 2):183-7. doi: 10.1042/0300-5127:0290183.
引用本文的文献
1
Human translesion DNA polymerases ι and κ mediate tolerance to temozolomide in MGMT-deficient glioblastoma cells.人类跨损伤 DNA 聚合酶 ι 和 κ 介导 MGMT 缺陷型胶质母细胞瘤细胞对替莫唑胺的耐受。
DNA Repair (Amst). 2024 Sep;141:103715. doi: 10.1016/j.dnarep.2024.103715. Epub 2024 Jul 18.
2
The levels of p53 govern the hierarchy of DNA damage tolerance pathway usage.p53的水平决定了DNA损伤耐受途径使用的层级关系。
Nucleic Acids Res. 2024 Apr 24;52(7):3740-3760. doi: 10.1093/nar/gkae061.
3
Polymerase iota (Pol ι) prevents PrimPol-mediated nascent DNA synthesis and chromosome instability.聚合酶 ι（Pol ι）可防止 PrimPol 介导的新生 DNA 合成和染色体不稳定性。
Sci Adv. 2023 Apr 14;9(15):eade7997. doi: 10.1126/sciadv.ade7997.
4
Beyond the Lesion: Back to High Fidelity DNA Synthesis.超越损伤：回归高保真DNA合成。
Front Mol Biosci. 2022 Jan 5;8:811540. doi: 10.3389/fmolb.2021.811540. eCollection 2021.
5
Identification and Characterization of Thermostable Y-Family DNA Polymerases η, ι, κ and Rev1 From a Lower Eukaryote, .来自低等真核生物的耐热Y家族DNA聚合酶η、ι、κ和Rev1的鉴定与表征
Front Mol Biosci. 2021 Nov 3;8:778400. doi: 10.3389/fmolb.2021.778400. eCollection 2021.
本文引用的文献
1
Polymerase iota - an odd sibling among Y family polymerases.聚合酶iota——Y 家族聚合酶中的一个奇异兄弟。
DNA Repair (Amst). 2020 Feb;86:102753. doi: 10.1016/j.dnarep.2019.102753. Epub 2019 Nov 20.
2
Translesion DNA polymerases in eukaryotes: what makes them tick?真核生物中的跨损伤DNA聚合酶：它们是如何工作的？
Crit Rev Biochem Mol Biol. 2017 Jun;52(3):274-303. doi: 10.1080/10409238.2017.1291576. Epub 2017 Mar 9.
3
Mouse DNA polymerase ι lacking the forty-two amino acids encoded by exon-2 is catalytically inactive in vitro.缺少外显子2编码的42个氨基酸的小鼠DNA聚合酶ι在体外没有催化活性。
DNA Repair (Amst). 2017 Feb;50:71-76. doi: 10.1016/j.dnarep.2016.12.004. Epub 2016 Dec 21.
4
DNA polymerase ι functions in the generation of tandem mutations during somatic hypermutation of antibody genes.DNA聚合酶ι在抗体基因体细胞超突变过程中串联突变的产生中发挥作用。
J Exp Med. 2016 Aug 22;213(9):1675-83. doi: 10.1084/jem.20151227. Epub 2016 Jul 25.
5
Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese.在锰存在的情况下，人类DNA聚合酶iota的催化活性增加且保真度改变。
J Biol Chem. 2007 Aug 24;282(34):24689-96. doi: 10.1074/jbc.M702159200. Epub 2007 Jul 2.
6
Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations.有证据表明，在缺乏DNA聚合酶η的着色性干皮病变异细胞中，DNA聚合酶ι会导致紫外线诱导的突变出现非常高的频率和独特的谱型。
Cancer Res. 2007 Apr 1;67(7):3018-26. doi: 10.1158/0008-5472.CAN-06-3073.
7
Normal hypermutation in antibody genes from congenic mice defective for DNA polymerase iota.来自DNA聚合酶ι缺陷的同源小鼠抗体基因的正常超突变。
DNA Repair (Amst). 2006 Mar 7;5(3):392-8. doi: 10.1016/j.dnarep.2005.12.006. Epub 2006 Jan 26.
8
DNA polymerase iota-dependent translesion replication of uracil containing cyclobutane pyrimidine dimers.DNA聚合酶ι介导的含尿嘧啶环丁烷嘧啶二聚体的跨损伤复制
DNA Repair (Amst). 2006 Feb 3;5(2):210-8. doi: 10.1016/j.dnarep.2005.09.011. Epub 2005 Nov 2.
9
Proliferating cell nuclear antigen-dependent coordination of the biological functions of human DNA polymerase iota.增殖细胞核抗原依赖的人类DNA聚合酶ι生物功能的协调作用
J Biol Chem. 2004 Nov 12;279(46):48360-8. doi: 10.1074/jbc.M406511200. Epub 2004 Sep 1.
10
Sequence context-dependent replication of DNA templates containing UV-induced lesions by human DNA polymerase iota.人DNA聚合酶iota对含有紫外线诱导损伤的DNA模板进行序列上下文依赖性复制。
DNA Repair (Amst). 2003 Sep 18;2(9):991-1006. doi: 10.1016/s1568-7864(03)00094-6.
Zotero 插件