Osoagbaka O U, Njoku-Obi A N
Department of Microbiology, University of Nigeria, Nsukka.
J Appl Bacteriol. 1987 Jul;63(1):27-38. doi: 10.1111/j.1365-2672.1987.tb02414.x.
Three hundred samples of sputum from patients suffering from various forms of pulmonary disorders were homogenized by pancreatic digestion, examined microscopically and cultured on brain heart infusion agar, trypticase soy agar, in brain heart infusion broth, trypticase soy broth and McClung's carbon-free broth. Several elements of varying morphological forms, believed to be from aerobic actinomycetes or nocardias, were observed in 16 cases. Six strains of Nocardia asteroides, one N. brasiliensis, eight of Rhodococcus species and one Micromonospora were isolated and studied at various stages of growth. Several nocardial and rhodococcal elements closely resembling those observed in the Gram-stained sputa were found. It is suggested that some of those in the sputum were from nocardias and that their appearance in sputum, as seen from the Gram-stained slide, when combined with clinical symptoms can serve for a presumptive diagnosis of pulmonary nocardiosis pending a more definitive identification by a specialized laboratory.
对300份患有各种形式肺部疾病患者的痰液样本进行胰酶消化均质化处理，进行显微镜检查，并在脑心浸液琼脂、胰蛋白酶大豆琼脂、脑心浸液肉汤、胰蛋白酶大豆肉汤和麦克伦无碳肉汤中培养。在16例病例中观察到几种形态各异的成分，据信来自需氧放线菌或诺卡氏菌。分离出6株星形诺卡氏菌、1株巴西诺卡氏菌、8株红球菌属菌株和1株小单孢菌，并在不同生长阶段进行研究。发现了几种与革兰氏染色痰液中观察到的成分非常相似的诺卡氏菌和红球菌成分。有人提出，痰液中的一些成分来自诺卡氏菌，从革兰氏染色载玻片上看到它们在痰液中的出现，结合临床症状，可在专业实验室进行更明确鉴定之前，用于肺部诺卡氏菌病的初步诊断。
