Wu Yue-Xia, Si Shan-Shan, Zhang Xin, Lian Ke-Qian
Stomatological Hospital， Southern Medical University. Guangzhou 510280, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2020 Aug;29(4):380-385.
To investigate the effect of testosterone level on inflammatory bone resorption in periodontitis with mice.
Forty-eight SD mice were randomly divided into unligated group, sham operation group, castration group, castration + testosterone group, 12 mice in each group. At 6 weeks after ligation, serum testosterone levels were measured, and alveolar bone loss (ABL) and alveolar bone absorption area were compared by hematoxylin - eosin staining and methylene blue staining. The expression of inflammatory cytokine messenger RNA(mRNA) in gingival tissue was measured by real-time fluorescence quantitative PCR. SPSS 20.0 software package was used to analyze the data.
Serum testosterone level among four groups was the highest in the unligated group, followed by castration + testosterone group, sham operation group and castration group, with significant difference(P<0.05). The ABL of the castration + testosterone group was significantly larger than that of the unligated group, the sham operation group and the castration group(P<0.05). The ABL of the castration group was significantly smaller than that of the sham operation group (P<0.05). The alveolar bone resorption area of the castration + testosterone group was significantly larger than that of the unligated group, the sham operation group and the castration group(P<0.05). The alveolar bone resorption area of the castration group was significantly smaller than that of the sham operation group (P<0.05). Interleukin-1β (IL-1β) mRNA, interleukin-6(IL-6) mRNA and tumor necrosis factor-α(TNF-α) mRNA levels in gingival tissues of sham operation group, castration group and castration + testosterone group were significantly higher than the unligated group. The levels of interleukin-10(IL-10) mRNA in gingival tissues of sham operation group, castration group and castration + testosterone group were significantly lower than those in unligated group(P<0.05). The level of IL-1β mRNA in gingival tissues among four groups was the the highest in the unligated group, followed by castration + testosterone group, sham operation group and castration group, with significant difference (P<0.05). Serum testosterone levels were positively correlated with ABL, alveolar bone resorption area, and IL-1β (P<0.05).
Periodontitis with mice have decreased testosterone levels, and long-term testosterone depletion can reduce inflammatory bone resorption in alveolar bone, which may be achieved by reducing the level of IL-1β, indicating that reduction of the level of testosterone in periodontitis patients may be a new treatment target for alveolar bone resorption.
研究睾酮水平对小鼠牙周炎炎症性骨吸收的影响。
将48只SD小鼠随机分为未结扎组、假手术组、去势组、去势+睾酮组，每组12只。结扎后6周，测定血清睾酮水平，通过苏木精-伊红染色和亚甲蓝染色比较牙槽骨吸收（ABL）和牙槽骨吸收面积。采用实时荧光定量PCR检测牙龈组织中炎性细胞因子信使核糖核酸（mRNA）的表达。使用SPSS 20.0软件包进行数据分析。
四组血清睾酮水平以未结扎组最高，其次为去势+睾酮组、假手术组、去势组，差异有统计学意义（P<0.05）。去势+睾酮组的ABL明显大于未结扎组、假手术组和去势组（P<0.05）。去势组的ABL明显小于假手术组（P<0.05）。去势+睾酮组的牙槽骨吸收面积明显大于未结扎组、假手术组和去势组（P<0.05）。去势组的牙槽骨吸收面积明显小于假手术组（P<0.05）。假手术组、去势组和去势+睾酮组牙龈组织中白细胞介素-1β（IL-1β）mRNA、白细胞介素-6（IL-6）mRNA和肿瘤坏死因子-α（TNF-α）mRNA水平均明显高于未结扎组。假手术组、去势组和去势+睾酮组牙龈组织中白细胞介素-10（IL-10）mRNA水平均明显低于未结扎组（P<0.05）。四组牙龈组织中IL-1β mRNA水平以未结扎组最高，其次为去势+睾酮组、假手术组、去势组，差异有统计学意义（P<0.05）。血清睾酮水平与ABL、牙槽骨吸收面积及IL-1β呈正相关（P<0.05）。
小鼠牙周炎时睾酮水平降低，长期睾酮缺乏可减少牙槽骨的炎症性骨吸收，可能是通过降低IL-1β水平实现的，提示降低牙周炎患者的睾酮水平可能是牙槽骨吸收的一个新的治疗靶点。
