Visvesvara G S, Peralta M J, Brandt F H, Wilson M, Aloisio C, Franko E
Division of Parasitic Diseases, Centers for Diseases Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1987 Sep;25(9):1629-34. doi: 10.1128/jcm.25.9.1629-1634.1987.
Monoclonal antibodies (MAbs) to Naegleria fowleri, the etiologic agent of primary amebic meningoencephalitis (PAM), have been produced and used as probes to identify N. fowleri amebae in brain sections of patients who died of that disease. These MAbs were characterized for their specificity by the indirect immunofluorescence assay (IIF), dot immunobinding assay (DIBA), and enzyme-linked immunotransfer blot technique (EITB). The MAbs reacted intensely with all strains of N. fowleri tested originating from different geographic areas in the IIF and DIBA tests, but showed no reactivity with four other species of Naegleria, N. gruberi, N. jadini, N. lovaniensis, and N. australiensis, or a strain of Acanthamoeba castellanii. In the EITB assay the MAbs reacted with the antigens of N. fowleri and produced intensely staining bands at the 160-, 104-, 93-, and 66-kilodalton (kDa) regions and several minor bands at the 30- and 50-kDa regions. The MAbs also reacted with the antigens of N. lovaniensis and produced a darkly staining band at 160 kDa and a diffusely staining band at 116 kDa, indicating that these antigens were shared by the two species. The MAbs, however, showed no reactivity with N. jadini and N. gruberi in the EITB assay.
针对原发性阿米巴脑膜脑炎（PAM）的病原体福氏耐格里阿米巴的单克隆抗体（MAbs）已被制备出来，并用作探针，以鉴定死于该疾病患者脑切片中的福氏耐格里阿米巴。通过间接免疫荧光测定法（IIF）、斑点免疫结合测定法（DIBA）和酶联免疫转移印迹技术（EITB）对这些单克隆抗体的特异性进行了表征。在IIF和DIBA试验中，这些单克隆抗体与来自不同地理区域的所有测试福氏耐格里阿米巴菌株发生强烈反应，但与其他四种耐格里阿米巴（格氏耐格里阿米巴、贾氏耐格里阿米巴、洛氏耐格里阿米巴和澳大利亚耐格里阿米巴）或一株卡氏棘阿米巴均无反应。在EITB试验中，这些单克隆抗体与福氏耐格里阿米巴的抗原发生反应，并在160、104、93和66千道尔顿（kDa）区域产生强烈染色带，在30和50 kDa区域产生几条次要带。这些单克隆抗体还与洛氏耐格里阿米巴的抗原发生反应，在160 kDa处产生一条深色染色带，在116 kDa处产生一条弥散染色带，表明这两个物种共享这些抗原。然而，在EITB试验中，这些单克隆抗体与贾氏耐格里阿米巴和格氏耐格里阿米巴无反应。
