Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, 46022 Valencia, Spain.
Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
Anal Chem. 2020 Nov 3;92(21):14608-14615. doi: 10.1021/acs.analchem.0c03020. Epub 2020 Oct 22.
β-lactams (BLCs) are the most widely used antibiotics and consequently the most common cause of drug allergy in the world. The diagnosis of drug allergy is complex and represents a serious challenge that includes a wide variety of methods. In vitro tests are based on the immunological determination of allergen-specific IgE, but the tests in the market lack the required sensitivity and specificity. In addition, the large sample volume, long incubation times, and single-plex configuration have brought their use into question to complement the clinical information. Here, we report a chemiluminescence immunoassay (CLIA) for multiparametric quantification of specific IgE to penicillin G, penicillin V, amoxicillin, and piperacillin, using histone H1 as a carrier. The developed CLIA allowed the determination of BLC-specific IgE below 0.1 IU/mL, thus allowing identification of allergic patients with better sensitivity, using only 25 μL of a sample (serum). The immunoassay was successfully applied in a cohort of 140 human serum samples, showing good sensitivity (64.6%) as well as specificity (100%), which significantly improve the predictive character of existing BLC-allergy in vitro tests.
β-内酰胺类抗生素（BLCs）是应用最广泛的抗生素，也是世界范围内最常见的药物过敏原因。药物过敏的诊断较为复杂，是一个严峻的挑战，涉及多种方法。体外检测基于过敏原特异性 IgE 的免疫学测定，但市场上的检测方法缺乏所需的灵敏度和特异性。此外，大样本量、长孵育时间和单重配置使其在补充临床信息方面的应用受到质疑。在此，我们报道了一种使用组蛋白 H1 作为载体的用于青霉素 G、青霉素 V、阿莫西林和哌拉西林特异性 IgE 多参数定量的化学发光免疫分析（CLIA）。开发的 CLIA 可在 0.1IU/mL 以下检测 BLC 特异性 IgE，从而仅使用 25μL 样本（血清）即可提高过敏患者的检测灵敏度。该免疫分析已成功应用于 140 个人类血清样本，其灵敏度（64.6%）和特异性（100%）均较好，显著提高了现有 BLC 过敏体外检测的预测能力。
