School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Anal Methods. 2020 Nov 21;12(43):5195-5201. doi: 10.1039/d0ay01702g. Epub 2020 Oct 22.
N-Doped carbon dots (CDs) had been simply produced by a one-pot synthesis process using amygdalic acid and threonine. The resulting product was water-soluble and exhibited strong luminescence emission with a fluorescence quantum yield of 19.25%. The emission of CDs was obviously and selectively decreased upon adding 2,4,6-trinitrophenol (TNP). It was proved that the fluorescence resonance energy transfer was the main mechanism for quenching. An efficient fluorescence probe with satisfied sensitivity for TNP determination was found. The range of the linear response for TNP detection was 0.5-40.0 μmol L, and the limit of detection was 20 nmol L. The content of trace TNP in water samples was successfully detected with this method. The CDs were also applied in HepG2 cell imaging and the fabrication of fluorescent films by dispersing the solid freeze-drying CD (SCD) powder into PMMA, which exhibited some application value in biology and photovoltaics.
氮掺杂碳点（CDs）通过杏仁酸和苏氨酸的一锅合成法简单制备。所得产物水溶性好，具有强的荧光发射，荧光量子产率为 19.25%。加入 2,4,6-三硝基苯酚（TNP）后，CDs 的发射明显且选择性降低。证明荧光共振能量转移是猝灭的主要机制。发现了一种用于 TNP 测定的灵敏度高的有效荧光探针。TNP 检测的线性响应范围为 0.5-40.0 μmol L，检出限为 20 nmol L。该方法成功检测了水样中痕量 TNP 的含量。CDs 还应用于 HepG2 细胞成像以及将固态冻干 CD（SCD）粉末分散到 PMMA 中制备荧光膜，在生物学和光伏领域具有一定的应用价值。
