Onoda T, Oshima A, Nakano S, Matsuno A
Department of Biology, Faculty of Science, Shimane University, Matsue, Japan.
J Gen Microbiol. 1987 Mar;133(3):527-34. doi: 10.1099/00221287-133-3-527.
An L-form isolated from Escherichia coli K12 by sequential treatment with N-methyl-N'-nitro-N-nitrosoguanidine and lysozyme was adapted to grow in hyperosmolar liquid cultures. It was stable in the absence of antibiotic when cultured in brain heart infusion (BHI) broth containing NaCl and CaCl2, the optimal concentrations being 0.34 M and 1 mM, respectively. No growth of the L-form was observed when CaCl2 was not added to BHI medium containing 0.34 M-NaCl. On the other hand, when KCl replaced NaCl as the osmotic stabilizer, growth of the L-form was repressed in the presence of CaCl2. Electron microscopy of the L-form confirmed the absence of a cell wall. A revertant strain derived from the L-form grew as a stable bacillary form in BHI medium without osmotic stabilizer. The growth characteristics of the revertant strain resembled those of the parent strain. The revertant strain produced L-forms in the presence of NaCl.
通过用N-甲基-N'-硝基-N-亚硝基胍和溶菌酶依次处理从大肠杆菌K12中分离出的L型菌，使其适应在高渗液体培养基中生长。当在含有NaCl和CaCl2的脑心浸液（BHI）肉汤中培养时，该L型菌在无抗生素的情况下是稳定的，最佳浓度分别为0.34 M和1 mM。当不向含有0.34 M NaCl的BHI培养基中添加CaCl2时，未观察到L型菌生长。另一方面，当用KCl替代NaCl作为渗透稳定剂时，在CaCl2存在下L型菌的生长受到抑制。L型菌的电子显微镜检查证实其没有细胞壁。从L型菌衍生的回复菌株在没有渗透稳定剂的BHI培养基中以稳定的杆菌形式生长。回复菌株的生长特性与亲本菌株相似。回复菌株在NaCl存在下产生L型菌。
