白细胞表面存在膜结合型CD99配体。

The presence of membrane bound CD99 ligands on leukocyte surface.

作者信息

Takheaw Nuchjira, Pata Supansa, Laopajon Witida, Roytrakul Sittiruk, Kasinrerk Watchara

机构信息

Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.

Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.

出版信息

BMC Res Notes. 2020 Oct 22;13(1):496. doi: 10.1186/s13104-020-05347-0.

DOI:10.1186/s13104-020-05347-0

PMID:33092634

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7583281/

Abstract

OBJECTIVE

CD99, a leukocyte surface molecule, reportedly plays an important role in several cellular processes. However, the role of CD99 in T cell regulation remains unclear, as the CD99 ligand associated with T-cell regulation has not yet been identified. Our previous study showed that recombinant CD99 bound to CD99 ligands was expressed on monocytes, NK cells and dendritic cells. This interaction regulates the expression of IL-6 and TNF-α in CD3 + T cells following T cell activation. In the present study, we confirmed the presence of CD99 ligands in immune cells.

RESULTS

A recombinant CD99-human IgG fusion protein, CD99HIgG, was produced and used to search for CD99 ligand expression in various hematopoietic cell lines. Among several cell lines, THP-1 monocytic cell line showed strong positive reaction for CD99HIgG, and CD99 and CD99 ligand complexes were pulled-down using a DTSSP cross-linker. The study demonstrated the presence of the membrane bound CD99 ligand, and CD99 ligand candidates were identified via LC-MS/MS. These results may be useful to further identify the CD99 ligands, and to fully comprehend the role of CD99 in immunoregulation.

摘要

目的

据报道，白细胞表面分子CD99在多个细胞过程中发挥重要作用。然而，CD99在T细胞调节中的作用仍不清楚，因为尚未鉴定出与T细胞调节相关的CD99配体。我们之前的研究表明，与CD99配体结合的重组CD99在单核细胞、自然杀伤细胞和树突状细胞上表达。这种相互作用调节T细胞活化后CD3 + T细胞中IL-6和TNF-α的表达。在本研究中，我们证实了免疫细胞中存在CD99配体。

结果

制备了重组CD99-人IgG融合蛋白CD99HIgG，并用于寻找各种造血细胞系中CD99配体的表达。在几种细胞系中，THP-1单核细胞系对CD99HIgG呈强阳性反应，并使用DTSSP交联剂下拉CD99和CD99配体复合物。该研究证明了膜结合CD99配体的存在，并通过LC-MS/MS鉴定了CD99配体候选物。这些结果可能有助于进一步鉴定CD99配体，并全面理解CD99在免疫调节中的作用。