Food Technology Department, College of Agriculture, Campus II, Can Tho University, Can Tho City, Vietnam.
National Agro - Forestry - Fishery Quality Assurance Department, Branch 6 (NAFIQUAD), Can Tho City, Vietnam.
J Food Biochem. 2020 Dec;44(12):e13533. doi: 10.1111/jfbc.13533. Epub 2020 Oct 22.
Germinated brown rice (GBR) consists of bioactive compounds (BCs) that are very useful for diabetes treatment. Modified GBR-based flour (MGBRF) was produced by modifying the starch in GBR with 0, 299.19, 598.38, and 897.57 U/ml of cyclodextrin glycosyltransferase (CGTase) for 1 hr and then spray-dried to examine its antidiabetic and cytotoxic effects. The results showed that the slowly digestible starch and resistant starch by modifying the starch in GBR with 598.38 U/ml of CGTase were 55.8% and 5.92% corresponding to the increase of γ-amino butyric acid (GABA) and ferulic acid (FA) with 4.31 ± 0.68 mg/ml and 3.10 ± 0.02 mg/ml, respectively. The extract from MGBRF showed strong cytotoxic capacity against HepG2. Furthermore, the in vivo study revealed the stability of the glycemic index (GI) by consuming MGBRF with significant impacts on diabetes. These results suggest that MGBRF through the action of CGTase plays a major role in antidiabetes and HepG2 cell product value addition. PRACTICAL APPLICATIONS: GBR consists of BCs that are useful for diabetes and cancer treatment. However, when using this or GBR-based products, it is difficult to evaluate the effect of functional properties, especially for diabetes and/or cancer diseases due to high starch content. Therefore, the modification of starch to limit digestible starch, increase SDS and RS as well as to enhance the effect of BCs on diabetes and cytotoxic activity on cancer cell should be studied before producing various based products from GBR. The results in this study indicated that CGTase increased BCs without any glycosides BCs in the extract. The MGBRF changed to higher RS and SDS while increasing the BCs. The extract of MGBRF showed strong cytotoxic activity against HepG2 cell and a positive effect on type 2-diabetic mice. Hence, this study produces new information for effective use of GBR-based food as a functional food.
发芽糙米（GBR）含有对糖尿病治疗非常有用的生物活性化合物（BC）。通过用 0、299.19、598.38 和 897.57 U/ml 的环糊精糖基转移酶（CGTase）修饰 GBR 中的淀粉 1 小时，然后喷雾干燥，制备改性 GBR 基面粉（MGBRF），以研究其抗糖尿病和细胞毒性作用。结果表明，用 598.38 U/ml 的 CGTase 修饰 GBR 中的淀粉后，慢消化淀粉和抗性淀粉分别增加到 55.8%和 5.92%，相应的γ-氨基丁酸（GABA）和阿魏酸（FA）分别增加到 4.31±0.68mg/ml 和 3.10±0.02mg/ml。MGBRF 的提取物对 HepG2 具有很强的细胞毒性。此外，体内研究表明，通过消耗 MGBRF 来稳定血糖指数（GI）对糖尿病有显著影响。这些结果表明，MGBRF 通过 CGTase 的作用在抗糖尿病和 HepG2 细胞产物增值方面发挥了重要作用。实际应用：GBR 含有对糖尿病和癌症治疗有用的 BC。然而，在使用这种或基于 GBR 的产品时，由于淀粉含量高，很难评估其功能特性的效果，特别是对糖尿病和/或癌症疾病。因此，在从 GBR 生产各种基于产品之前，应该研究淀粉的改性以限制可消化淀粉，增加 SDS 和 RS 以及增强 BCs 对糖尿病和癌细胞的细胞毒性作用。本研究结果表明，CGTase 增加了提取物中没有任何糖苷 BCs 的 BCs。MGBRF 在增加 BCs 的同时转变为更高的 RS 和 SDS。MGBRF 的提取物对 HepG2 细胞具有很强的细胞毒性活性，并对 2 型糖尿病小鼠有积极作用。因此，本研究为有效利用基于 GBR 的食品作为功能性食品提供了新的信息。
