胃癌、十二指肠溃疡及与幽门螺杆菌相关的非溃疡性消化不良患者中cagL与cagA、vacAs-m、babA基因之间的关联。

The association between cagL and cagA, vacAs-m, babA genes in patients with gastric cancer, duodenal ulcer, and non-ulcer dyspepsia related to Helicobacter pylori.

作者信息

Demiryas S, Caliskan R, Saribas S, Akkus S, Gareayaghi N, Kirmusaoglu S, Kepil N, Dinc H, Dag H, Dagdeviren E, Tokman H B, Kalayci F, Demirci M, Tasci I, Erzin Y, Bal K, Kocazeybek B

机构信息

Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of General Surgery, Istanbul, Turkey.

Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey.

出版信息

Acta Gastroenterol Belg. 2020 Jul-Sep;83(3):385-392.

PMID:33094584

Abstract

INTRODUCTION

As a component of the cag T4SS, the cagL gene is involved in the translocation of CagA into host cells and is essential for the formation of cag PAI-associated pili between H. pylori and gastric epithelial cells.

AIM

We aimed to investigate the clinical association of the cagL gene with other virulence factors (VacA, CagA, EPIYA-C, and BabA protein) of H. pylori strains isolated from GC, duodenal ulcer (DU), and non-ulcer dyspepsia (NUD) cases.

METHODS

The patient group (PG), including 47 patients (22 GC and 25 DU) and a 25 control group (CG= NUD) were included. Amplification of the H. pylori cagL, cagA, vacA, and babA2 genes and typing of EPIYA motifs were performed by PCR methods.

RESULTS

Sixty-one (84.7%) H. pylori strains were detected with cagL (93.6% in SG, 68% in CG). We detected a significant difference between SG and CG for the presence of cagL (p=0.012) but no statistical comparison was done for (≥2) EPIYA-C repeats In the comparison of H. pylori strains with cagA/vacAs1m1 and cagA/ vacAs1m2 and babA2 for the presence of cagL, we could not detect a significant difference (p=1).

CONCLUSION

We detected a significant difference between groups for the presence of cagL genotype (p=0.012). The vacAs1m1 (OR: 2.829), genotypes increased the GC and DU risk by 2.8 times, while multiple (≥2) EPIYA-C repeats incresed the GC and DU risk by 3.524 times. Gender (to be female) (OR: 0.454) decreased the GC and DU risk by inversly decreased in the multivariate analysis.

摘要

引言

作为cag T4SS的一个组成部分，cagL基因参与CagA转运至宿主细胞，并且对于幽门螺杆菌与胃上皮细胞之间cag PAI相关菌毛的形成至关重要。

目的

我们旨在研究cagL基因与从胃癌（GC）、十二指肠溃疡（DU）和非溃疡性消化不良（NUD）病例中分离出的幽门螺杆菌菌株的其他毒力因子（VacA、CagA、EPIYA-C和BabA蛋白）之间的临床关联。

方法

纳入患者组（PG），包括47例患者（22例GC和25例DU）以及一个25例的对照组（CG = NUD）。采用PCR方法对幽门螺杆菌的cagL、cagA、vacA和babA2基因进行扩增，并对EPIYA基序进行分型。

结果

检测到61株（84.7%）幽门螺杆菌菌株携带cagL（SG组中为93.6%，CG组中为68%）。我们检测到SG组和CG组在cagL存在情况上有显著差异（p = 0.012），但对于（≥2）个EPIYA-C重复序列未进行统计学比较。在比较携带cagA/vacAs1m1和cagA/vacAs1m2以及babA2的幽门螺杆菌菌株中cagL的存在情况时，我们未检测到显著差异（p = 1）。