Department of Dentistry and Christus University Center (Unichristus), Fortaleza-CE, Brazil.
Department of Dentistry Sciences, Christus University Center (Unichristus), Fortaleza-CE, Brazil.
Photobiomodul Photomed Laser Surg. 2020 Nov;38(11):673-678. doi: 10.1089/photob.2020.4844. Epub 2020 Oct 23.
We evaluated the influence of photobiomodulation therapy (PBMT) application during bone healing postexodontia in rats. We divided 84 male Wistar rats into a control group (CG), which received placebo treatment without PBMT, and a test group (TG), which was treated with PBMT. After exodontia, the animals were subjected to PBMT (TG) with an AsGaAI diode laser at 810 nm, 100 mW, 2 J, and 70 J/cm or placebo treatment (CG) every 72 h. After 1, 3, 7, 14, 21, and 28 days, the animals were weighed and euthanized to remove the left hemimandibles for radiographic (alveolar filling) and histomorphometric (inflammatory polymorphonuclear cell (PMN), mononuclear cell (MN), osteoclast (OC), and blood vessels counting) analysis. Statistic approach used two-way variance analysis followed by Bonferroni post hoc ( < 0.05, GraphPad Prism 5.0). There was no significant difference in body mass variation ( = 0.828) and bone neoformation ( = 0.365) between the two groups, but the TG presented lower PMN ( < 0.001), MN ( < 0.001), and OC counts ( < 0.001) and higher blood vessels count ( < 0.001) throughout the repair process. PBMT attenuated the inflammatory process after exodontia without interfering with bone neoformation.
我们评估了光生物调节疗法（PBMT）在拔牙后骨愈合中的应用对大鼠的影响。我们将 84 只雄性 Wistar 大鼠分为对照组（CG）和实验组（TG）。CG 组接受安慰剂治疗，不进行 PBMT，TG 组则接受 PBMT。拔牙后，动物接受 810nm、100mW、2J 和 70J/cm 的 AsGaAI 二极管激光 PBMT（TG）或安慰剂治疗（CG），每 72 小时一次。在第 1、3、7、14、21 和 28 天，称重并处死动物，以去除左侧下颌骨进行放射学（牙槽填充）和组织形态计量学（炎症多形核细胞（PMN）、单核细胞（MN）、破骨细胞（OC）和血管计数）分析。采用双向方差分析，随后进行 Bonferroni 事后检验（ < 0.05，GraphPad Prism 5.0）。两组动物的体重变化（ = 0.828）和骨新生（ = 0.365）无显著差异，但 TG 在整个修复过程中PMN（ < 0.001）、MN（ < 0.001）和 OC 计数（ < 0.001）较低，而血管计数（ < 0.001）较高。PBMT 减轻了拔牙后的炎症过程，而不影响骨新生。
