Vihko P, Kontturi M, Lukkarinen O, Martikainen P, Pelliniemi L, Heikkilä J, Vihko R
Biocenter, University of Oulu, Finland.
Prostate. 1987;11(1):51-7. doi: 10.1002/pros.2990110107.
Five patients who were candidates for radical prostatectomy were investigated. One milligram of diethylene triamine pentaacetic acid derivatives of purified F(ab')2 fragments of monoclonal antibodies against prostatic acid phosphatase, labeled with 99mTc, were bilaterally injected into the periprostatic space. The amount of radioactivity injected varied between 3 and 7 mCi. Imaging took place dynamically for the first hour following injection, then at 6 and 24 hr. In one patient, lymph node metastases were detected in the left paraaortic, iliac, and obturator lymph nodes by this technique. The lesions incorporating radioactivity were confirmed to be metastases of prostatic cancer following staging pelvic lymphadenectomy. Immunolabeling electron microscopy studies revealed internalized antibody in prostatic cancer cells. In the four other patients, radioimaging did not show any lymph node metastases, and this negative finding was confirmed at surgery. These early data indicate the possibility of preoperative staging of prostatic cancer using radiolabeled derivatives of monoclonal antibodies raised against prostatic acid phosphatase and injected into the periprostatic area.
对5例适合行根治性前列腺切除术的患者进行了研究。将1毫克用99mTc标记的抗前列腺酸性磷酸酶单克隆抗体纯化F(ab')2片段的二乙三胺五乙酸衍生物双侧注入前列腺周围间隙。注入的放射性活度在3至7毫居里之间。注射后的第一个小时进行动态成像，然后在6小时和24小时进行成像。在1例患者中，通过该技术在左主动脉旁、髂和闭孔淋巴结中检测到淋巴结转移。在进行盆腔淋巴结清扫分期后，证实摄取放射性的病变为前列腺癌转移灶。免疫标记电子显微镜研究显示前列腺癌细胞中有内化抗体。在其他4例患者中，放射性成像未显示任何淋巴结转移，手术证实了这一阴性结果。这些早期数据表明，使用针对前列腺酸性磷酸酶的单克隆抗体的放射性标记衍生物并注入前列腺周围区域，有可能对前列腺癌进行术前分期。
