Diabetes Research Center (DRC), Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), Doha, Qatar.
Division of Research, Weill Cornell Medicine-Qatar, Qatar Foundation, Education City, Doha, Qatar.
Front Endocrinol (Lausanne). 2020 Sep 30;11:571357. doi: 10.3389/fendo.2020.571357. eCollection 2020.
Small noncoding microRNA (miRNA) have regulatory functions in polycystic ovary syndrome (PCOS) that differ to those in women without PCOS. However, little is known about miRNA expression in women with PCOS who are not insulin resistant (IR).
Circulating miRNAs were measured using quantitative polymerase chain reaction (qPCR) in 24 non-obese BMI and age matched women with PCOS and 24 control women. A miRNA data set was used to determine miRNA levels.
Women with PCOS showed a higher free androgen index (FAI) and anti-mullerian hormone (AMH) but IR did not differ. Four miRNAs (miR-1260a, miR-18b-5p, miR-424-5p, and miR let-7b-3p) differed between control and PCOS women that passed the false discovery rate (FDR) out of a total of 177 circulating miRNAs that were detected. MiRNA let-7b-3p correlated with AMH in PCOS (p < 0.05). When the groups were combined, miR-1260a correlated with FAI and let-7b-3p correlated with body mass index (BMI) (p < 0.05). There was no correlation to androgen levels. Ingenuity pathway analysis showed that nine of the top 10 miRNAs reported were associated with inflammatory pathways.
When IR did not differ between PCOS and control women, only four miRNA differed significantly suggesting that IR may be a driver for many of the miRNA changes reported. Let-7b-3p was related to AMH in PCOS, and to BMI as a group, whilst miR-1260a correlated with FAI. Androgen levels, however, had no effect upon circulating miRNA profiles. The expressed miRNAs were associated with the inflammatory pathway involving TNF and IL6.
小型非编码 microRNA（miRNA）在多囊卵巢综合征（PCOS）中具有调节功能，与非 PCOS 女性中的功能不同。然而，对于非胰岛素抵抗（IR）的 PCOS 女性的 miRNA 表达知之甚少。
使用定量聚合酶链反应（qPCR）测量 24 名非肥胖 BMI 和年龄匹配的 PCOS 女性和 24 名对照女性的循环 miRNA。使用 miRNA 数据集确定 miRNA 水平。
PCOS 女性的游离雄激素指数（FAI）和抗苗勒管激素（AMH）较高，但 IR 无差异。在总共检测到的 177 种循环 miRNA 中，有 4 种 miRNA（miR-1260a、miR-18b-5p、miR-424-5p 和 miR let-7b-3p）在对照和 PCOS 女性之间存在差异，这些差异通过了错误发现率（FDR）。miRNA let-7b-3p 与 PCOS 中的 AMH 相关（p<0.05）。当将两组结合时，miR-1260a 与 FAI 相关，let-7b-3p 与体重指数（BMI）相关（p<0.05）。与雄激素水平无关。Ingenuity 通路分析显示，报告的前 10 个 miRNA 中有 9 个与炎症通路相关。
当 PCOS 和对照女性之间的 IR 没有差异时，只有 4 种 miRNA 有显著差异，这表明 IR 可能是许多报道的 miRNA 变化的驱动因素。在 PCOS 中，let-7b-3p 与 AMH 相关，与 BMI 作为一个组相关，而 miR-1260a 与 FAI 相关。然而，雄激素水平对循环 miRNA 谱没有影响。表达的 miRNA 与涉及 TNF 和 IL6 的炎症通路相关。
