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gga-miR-200b-3p 通过靶向单核细胞向巨噬细胞分化相关基因促进 HD11 细胞中巨噬细胞的激活和分化。

gga-miR-200b-3p Promotes Macrophage Activation and Differentiation Targeting Monocyte to Macrophage Differentiation-Associated in HD11 Cells.

机构信息

College of Animal Science, South China Agricultural University, Guangzhou, China.

Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China.

出版信息

Front Immunol. 2020 Sep 30;11:563143. doi: 10.3389/fimmu.2020.563143. eCollection 2020.

DOI:10.3389/fimmu.2020.563143
PMID:33101281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7555432/
Abstract

MicroRNAs (miRNAs) play a critical role in various biological processes through regulation of gene expression post-transcriptionally. Although miRNAs are involved in cell proliferation and differentiation in mammals, few reports regarding the effects of host miRNAs on macrophage activation and differentiation are available in birds. Here, we reported that gga-miR-200b-3p acts as a positive regulator, enhancing macrophage activation and differentiation using an avian model. We found that ectopic expression of gga-miR-200b-3p in HD11 cells enhances the amount of MHC-II-positive cells and promotes the expression of pro-inflammatory cytokines and that gga-miR-200b-3p directly targets monocyte to macrophage differentiation-associated (MMD). The inhibition of MMD by gga-miR-200b-3p enhances the activation and differentiation of HD11 cells and increases the expression of pro-inflammatory cytokines. Collectively, these findings highlight a crucial role of gga-miR-200b-3p in macrophage activation and differentiation in birds.

摘要

微小 RNA（miRNAs）通过转录后调控基因表达，在各种生物学过程中发挥着关键作用。尽管 miRNAs 参与了哺乳动物的细胞增殖和分化，但鸟类中关于宿主 miRNAs 对巨噬细胞激活和分化影响的报道很少。在这里，我们报道了gga-miR-200b-3p 作为一个正调节剂，使用鸟类模型增强巨噬细胞的激活和分化。我们发现，gga-miR-200b-3p 在 HD11 细胞中的异位表达增加了 MHC-II 阳性细胞的数量，并促进了促炎细胞因子的表达，并且 gga-miR-200b-3p 直接靶向单核细胞向巨噬细胞分化相关（MMD）。gga-miR-200b-3p 对 MMD 的抑制增强了 HD11 细胞的激活和分化，并增加了促炎细胞因子的表达。总之，这些发现强调了 gga-miR-200b-3p 在鸟类巨噬细胞激活和分化中的重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/01cd3b7e6c84/fimmu-11-563143-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/05309408c557/fimmu-11-563143-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/ae0e696f0e39/fimmu-11-563143-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/c98c765d980a/fimmu-11-563143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/da8d9ba77b68/fimmu-11-563143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/b641208ebaeb/fimmu-11-563143-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/7d9c0891edb6/fimmu-11-563143-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/b19fb47c499c/fimmu-11-563143-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/01cd3b7e6c84/fimmu-11-563143-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/05309408c557/fimmu-11-563143-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/ae0e696f0e39/fimmu-11-563143-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/631b6fee1757/fimmu-11-563143-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/c98c765d980a/fimmu-11-563143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/da8d9ba77b68/fimmu-11-563143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/b641208ebaeb/fimmu-11-563143-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/7d9c0891edb6/fimmu-11-563143-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/b19fb47c499c/fimmu-11-563143-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cbd/7555432/01cd3b7e6c84/fimmu-11-563143-g009.jpg

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