Abreu-Naranjo Reinier, Paredes-Moreta Jonathan G, Granda-Albuja Genoveva, Iturralde Gabriel, González-Paramás Ana M, Alvarez-Suarez José M
Departamento de Ciencias de la Vida, Universidad Estatal Amazónica, Puyo, 160150, Ecuador.
Carrera de Biotecnología de los Recursos Naturales, Universidad Politécnica Salesiana, Quito, 170525, Ecuador.
Heliyon. 2020 Oct 16;6(10):e05211. doi: 10.1016/j.heliyon.2020.e05211. eCollection 2020 Oct.
The bioactive compounds, total antioxidant capacity and protective effect against lipid oxidative damage of red blood cell (RBC) membranes of L. extracts from three altitude levels (low, middle and high zones) were analyzed. The identification of the main polyphenols in the fruit was performed using High Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry analysis, while High-Performance Liquid Chromatography - Diode Array Detector was applied for the vitamin C and carotenoid analysis. The ability of the extracts (methanolic, free polyphenols and bound polyphenols) to protect RBC membranes against oxidative destruction was assessed utilizing the thiobarbituric acid reactive substance assay (TBARS) assay. Among the 14 phenolic compounds identified, quercetin glycosides were the most predominant ones. The fruit from the middle altitude zone showed higher vitamin C and lutein contents than those from the low and high areas, whilst higher values of -Carotene were obtained in L. from the low zone. The contents of flavonoids and total polyphenols were lower in fruit from the low zone, whilst the fruit from the middle zone had the highest values. Similar results were observed in the results of the antioxidant capacity assays. However, a significant difference was not found in the protective effect of the extracts from the three altitude zones against lipid peroxidation in RBC membranes.
对来自三个海拔高度区域（低、中、高地带）的枸杞提取物的生物活性化合物、总抗氧化能力以及对红细胞（RBC）膜脂质氧化损伤的保护作用进行了分析。使用高效液相色谱/电喷雾电离串联质谱分析法对果实中的主要多酚进行鉴定，同时采用高效液相色谱 - 二极管阵列检测器进行维生素C和类胡萝卜素分析。利用硫代巴比妥酸反应物（TBARS）测定法评估提取物（甲醇提取物、游离多酚和结合多酚）保护RBC膜免受氧化破坏的能力。在鉴定出的14种酚类化合物中，槲皮素糖苷是最主要的。中海拔区域的果实维生素C和叶黄素含量高于低海拔和高海拔区域的果实，而低海拔区域的枸杞β - 胡萝卜素含量更高。低海拔区域果实的黄酮类化合物和总多酚含量较低，而中海拔区域果实的含量最高。抗氧化能力测定结果也观察到类似结果。然而，三个海拔区域的提取物对RBC膜脂质过氧化的保护作用未发现显著差异。
