上调的 CNTN1 与结直肠癌的淋巴结转移和不良预后相关。

Upregulated CNTN1 is associated with lymph node metastasis and poor prognosis of colorectal cancer.

机构信息

Department of Gastrointestinal Surgery, The Second People's Hospital of Wuhu, Wuhu, Anhui, China.

Department of Trauma Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

出版信息

Cancer Biomark. 2021;30(2):193-201. doi: 10.3233/CBM-190981.

DOI:10.3233/CBM-190981

PMID:33104020

Abstract

BACKGROUND

Contactin1 (CNTN1), a member of the immunoglobulin superfamily, is known to correlate with tumor development and progression. Although recent studies have found that elevated CNTN1 has been demonstrated in some types of cancers, the expression and prognosis of CNTN1 in colorectal cancer (CRC) are unclear. Here, we aimed to determine the clinicopathological characteristics and prognostic role of CNTN1 in CRC patients.

METHODS

The protein expression of CNTN1 in tumor tissues was evaluated by immunohistochemistry. In addition, the mRNA and protein expressions of CNTN1 were examined by qRT-PCR and Western blotting analysis in 40 matched adjacent normal mucosa samples. The relationships of CNTN1 with clinicopathological data and prognosis significance were analyzed.

RESULTS

Immunohistochemical consequence suggested that the protein level of CNTN1 was obviously raised in CRC compared with adjacent normal mucosa tissues (56.9% vs 10.3%, P< 0.05). In addition, we detected a significant increase in CNTN1 mRNA and protein levels in CRC tissues compared with the matched adjacent normal mucosa tissues. Moreover, increased CNTN1 exprssion was significantly associated with tumor size, lymph node metastasis (LNM), tumor node-metastasis (TNM) stage and carcino-embryonic antigen (CEA) in clinical analysis. Kaplan-Meier analysis suggested that patients with CNTN1 over-expression showed worse overall survival (OS) (P= 0.001). Multivariate analysis indicated that high CNTN1 expression was an independent predictor for poor OS in CRC patients (P= 0.028). Further analysis revealed that patients with high CNTN1 combined with LNM present accurately predicted poorer outcome.

CONCLUSION

Taken together, the findingsindicate that CNTN1 plays a significant role and serve as a potential biomarker for the prediction of adverse prognosis in CRC. Intriguingly, high express of CNTN1 + LNM-present combination may improve the accuracy of prognosis.

摘要

背景

神经细胞黏附分子 1（Contactin1，CNTN1）是免疫球蛋白超家族的成员，已知与肿瘤的发生和发展相关。尽管最近的研究发现，一些类型的癌症中 CNTN1 的水平升高，但 CNTN1 在结直肠癌（CRC）中的表达和预后尚不清楚。在这里，我们旨在确定 CNTN1 在 CRC 患者中的临床病理特征和预后作用。

方法

通过免疫组织化学评估肿瘤组织中 CNTN1 的蛋白表达。此外，在 40 对匹配的相邻正常黏膜样本中，通过 qRT-PCR 和 Western blot 分析检测 CNTN1 的 mRNA 和蛋白表达。分析 CNTN1 与临床病理数据和预后意义的关系。

结果

免疫组化结果表明，CRC 组织中 CNTN1 蛋白水平明显高于相邻正常黏膜组织（56.9%比 10.3%，P<0.05）。此外，我们检测到 CRC 组织中 CNTN1 mRNA 和蛋白水平显著升高。此外，临床分析显示，CNTN1 表达增加与肿瘤大小、淋巴结转移（LNM）、肿瘤淋巴结转移（TNM）分期和癌胚抗原（CEA）显著相关。Kaplan-Meier 分析表明，CNTN1 过表达的患者总生存期（OS）更差（P=0.001）。多因素分析表明，CNTN1 高表达是 CRC 患者 OS 不良的独立预测因素（P=0.028）。进一步分析表明，CNTN1 高表达与 LNM 相结合的患者具有更差的预后。