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复合饲料中高比例黑麦对降低实验感染幼猪体内鼠伤寒沙门氏菌的作用

Effect of a High Proportion of Rye in Compound Feed for Reduction of Typhimurium in Experimentally Infected Young Pigs.

作者信息

Chuppava Bussarakam, Wilke Volker, Hartung Clara Berenike, El-Wahab Amr Abd, Grone Richard, von Felde Andreas, Kamphues Josef, Visscher Christian

机构信息

Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173 Hanover, Germany.

Department of Nutrition and Nutritional Deficiency Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.

出版信息

Microorganisms. 2020 Oct 22;8(11):1629. doi: 10.3390/microorganisms8111629.

Abstract

Public health concerns and the potential for food-borne zoonotic transmission have made a subject of surveillance programs in food-producing animals. Forty-two piglets (25 d of age and initially 7.48 kg) were used in a 28 d infection period to evaluate the effects of a high proportion of rye on reducing Typhimurium. Piglets were divided into two diet groups: control diet (wheat 69%) and experimental diet (rye 69%). After a one-week adaptation period, all piglets were orally infected with Typhimurium (10 log CFU/mL; 2mL/pig). in fecal shedding were evaluated at day 1, 3, 5, 7 and then weekly after infection. At the end of the experimental period (at day 28 after infection), the piglets were euthanized to sample feces, cecal digesta contents and ileocecal lymph nodes to determine the bacterial counts of . The results suggest that the bacterial counts in the experimental group fed rye diets showed evidence of reducing fecal shedding from day 14 onwards and decreasing the number of in cecal digesta. However, the translocation of in ileocecal lymph nodes was not affected. Furthermore, feed intake, weight gain and feed conversion did not differ between the groups ( > 0.05).

摘要

公共卫生问题以及食源性人畜共患病传播的可能性已成为产肉动物监测计划的一个主题。在为期28天的感染期内,使用了42头仔猪(25日龄,初始体重7.48千克)来评估高比例黑麦对减少鼠伤寒沙门氏菌的影响。仔猪被分为两个日粮组:对照日粮(小麦69%)和试验日粮(黑麦69%)。经过一周的适应期后,所有仔猪经口感染鼠伤寒沙门氏菌(10 log CFU/mL;每头猪2mL)。在感染后的第1、3、5、7天评估粪便排菌情况,之后每周评估一次。在试验期结束时(感染后第28天),对仔猪实施安乐死以采集粪便、盲肠消化物内容物和回盲部淋巴结样本,以确定鼠伤寒沙门氏菌的细菌计数。结果表明,饲喂黑麦日粮的试验组细菌计数显示,从第14天起粪便排菌有减少的迹象,盲肠消化物中的鼠伤寒沙门氏菌数量也有所减少。然而,回盲部淋巴结中鼠伤寒沙门氏菌的易位未受影响。此外,两组之间的采食量、体重增加和饲料转化率没有差异(P>0.05)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20aa/7690436/7353b88017c1/microorganisms-08-01629-g001.jpg

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