McGovern J, Crocker J
Department of Histopathology, East Birmingham Hospital, Bordesley Green East, England.
Am J Clin Pathol. 1987 Oct;88(4):480-3. doi: 10.1093/ajcp/88.4.480.
With the increasing use of immunoperoxidase technics, it may be difficult to differentiate between the dark staining of 3,3'-diaminobenzidine (DAB) compound reaction product and melanin pigment. The latter may be particularly observed in skin. Samples of both normal skin and melanotic malignant melanoma were treated for the removal of melanin by standard technics both before and after a peroxidase-antiperoxidase (PAP) immunohistologic sequence. Many methods for the removal of melanin pigment resulted in diminished DAB staining intensity. Some also caused cellular disruption. However, the method of choice was found to be treatment with 0.25 g/dL potassium permanganate and 1 g/dL oxalic acid before the immunoperoxidase sequence.
随着免疫过氧化物酶技术的使用日益增加，可能难以区分3,3'-二氨基联苯胺（DAB）复合反应产物的深色染色与黑色素。后者在皮肤中可能尤为明显。正常皮肤和黑素性恶性黑色素瘤样本在过氧化物酶-抗过氧化物酶（PAP）免疫组织学序列之前和之后均采用标准技术处理以去除黑色素。许多去除黑色素的方法导致DAB染色强度降低。有些还会引起细胞破坏。然而，发现首选方法是在免疫过氧化物酶序列之前用0.25 g/dL高锰酸钾和1 g/dL草酸处理。
