Research and Development, Australian Red Cross Lifeblood, Brisbane, QLD, Australia.
Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia.
Vox Sang. 2021 May;116(5):524-532. doi: 10.1111/vox.13020. Epub 2020 Oct 26.
Sheep are increasingly being used as a large in vivo animal model of blood transfusion because they provide several advantages over small animals. Understanding the effects of storage duration on ovine (ov) red cell concentrates (RCCs) and how these changes compare with stored human (hu) RCCs is necessary to facilitate clinical translation of research findings.
OvRCCs (n = 5) collected and processed in standard human blood collection packs, and equivalent huRCCs provided by Australian Red Cross Lifeblood (n = 5), were stored at 2-6°C for 42 days, with samples collected weekly. Haemolysis index was determined by measuring supernatant haemoglobin concentration. Biochemical parameters were evaluated using a blood gas analyser. Energy metabolites and biologically active lipids were measured using commercial assays. Osmotic fragility was determined by lysis in various saline concentrations. Extracellular vesicles were characterized by nanoparticle tracking analysis.
Ovine red blood cells (RBCs) are double in number, smaller in size and more fragile than human RBCs. Haematological values were unchanged throughout storage. In contrast, biochemical and metabolic values, and haemolysis index in three of the five ovRCCs exceeded huRCCs licensing criteria by day 42. Accumulation of extracellular vesicles and biologically active lipids was comparable between huRCCs and ovRCCs.
This study documents similarities and differences in the storage lesion of ovRCCs and huRCCs. This new information will guide the design of ovine transfusion models to enhance translation of findings to human transfusion settings.
绵羊越来越多地被用作输血的大型活体动物模型，因为它们相对于小动物具有多种优势。了解储存时间对绵羊（ov）红细胞浓缩物（RCC）的影响，以及这些变化如何与储存的人类（hu）RCC 相比，对于促进研究结果向临床转化是必要的。
从标准人类采血袋中收集并处理的 ovRCC（n=5）和澳大利亚红十字会生命血（Australian Red Cross Lifeblood）提供的等量 huRCC（n=5）在 2-6°C 下储存 42 天，每周采集一次样本。通过测量上清液血红蛋白浓度来确定溶血指数。使用血气分析仪评估生化参数。使用商业测定法测量能量代谢物和生物活性脂质。通过在不同盐浓度下溶解来确定渗透脆性。通过纳米颗粒跟踪分析对细胞外囊泡进行表征。
绵羊红细胞（RBC）的数量是人类 RBC 的两倍，体积更小，更脆弱。储存过程中血液学值保持不变。相比之下，在五份 ovRCC 中有三份的生化和代谢值以及溶血指数在第 42 天超过了 huRCC 许可标准。huRCC 和 ovRCC 之间细胞外囊泡和生物活性脂质的积累相似。
本研究记录了 ovRCC 和 huRCC 储存损伤的异同。这些新信息将指导绵羊输血模型的设计，以增强对人类输血环境的研究发现的转化。
