Suman Prachi, Manju R, Shetty Veena A, Hegde Amitha M, Rao Shama
NITTE (Deemed to be University), AB Shetty Memorial Institute of Dental Sciences, Department of Pediatric and Preventive Dentistry, Deralakatte, Mangalore, Karnataka, India.
Indian J Dent Res. 2020 Jul-Aug;31(4):562-568. doi: 10.4103/ijdr.IJDR_93_19.
Dental tissue remains are the toughest, and chemically, the most stable tissue in the body. Its high resilience in the events of fire and bacterial decomposition makes them vital for DNA analysis by PCR method.
Determination of sex of children through molecular analysis of pulp tissue of exfoliated deciduous teeth stored in different media and analyzed after a different time period.
Sixty samples of deciduous teeth were divided into three groups. Group IA and Group IIA were stored in natural soil and wet clay for 1 month, respectively. Group IB and Group IIB were stored in natural soil and wet clay for 6 months, respectively. Group III was analyzed immediately after extraction.
Sex determination was carried out in five steps: Pulp tissue removal, DNA isolation, DNA quantification, PCR amplification, Sex determination. X and Y specific chromosomes from each sample were amplified and compared.
Kruskal-Wallis test, Dunn's test, and Wilcoxon signed rank test.
Group III revealed the highest amount of DNA quantified. Amount of DNA quantified after 6 months of storage in natural soil and wet clay decreased in both the groups with the samples stored in wet clay showing a maximum decrease. Results of the PCR analysis also showed 100% accuracy rate in the samples of Group III.
Sex determination from pulp tissue depends a lot on the quality and quantity of DNA extracted. Sex could be effectively determined among the samples evaluated immediately after extraction. This ability decreases as the storage condition changes and the time period increases. Samples stored in wet clay were found to show the least sex identification ability than dry soil.
牙齿组织残骸是人体中最坚韧且化学性质最稳定的组织。其在火灾和细菌分解情况下的高复原性使其对于通过聚合酶链反应（PCR）方法进行DNA分析至关重要。
通过对储存在不同介质中并在不同时间段后进行分析的乳牙牙髓组织进行分子分析来确定儿童的性别。
60颗乳牙样本被分为三组。IA组和IIA组分别储存在天然土壤和湿黏土中1个月。IB组和IIB组分别储存在天然土壤和湿黏土中6个月。III组在拔牙后立即进行分析。
性别鉴定分五步进行：牙髓组织去除、DNA分离、DNA定量、PCR扩增、性别鉴定。对每个样本的X和Y特异性染色体进行扩增并比较。
Kruskal-Wallis检验、Dunn检验和Wilcoxon符号秩检验。
III组显示出定量的DNA量最高。在天然土壤和湿黏土中储存6个月后定量的DNA量在两组中均减少，其中储存在湿黏土中的样本减少最多。PCR分析结果在III组样本中也显示出100%的准确率。
从牙髓组织进行性别鉴定很大程度上取决于提取的DNA的质量和数量。在拔牙后立即评估的样本中可以有效地确定性别。随着储存条件的变化和时间的增加，这种能力会下降。发现储存在湿黏土中的样本比干燥土壤中的样本显示出最低的性别鉴定能力。
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