Department of Paediatric and Preventive Dentistry, AB Shetty Memorial Institute of Dental Sciences, NITTE Deemed to be University, Mangalore, Karnataka, India.
Department of Orthodontics and Dentofacial Orthopaedics, AB Shetty Memorial Institute of Dental Sciences, NITTE Deemed to be University, Mangalore, Karnataka, India.
Indian J Dent Res. 2022 Apr-Jun;33(2):158-163. doi: 10.4103/ijdr.ijdr_507_21.
The present study aimed to evaluate the effectiveness of pulp tissue collected from deciduous teeth for the determination of gender using polymerase chain reaction (PCR).
140 extracted deciduous teeth were selected. The control group comprised 20 teeth that were subjected to DNA analysis immediately. Whereas Group I and Group II consisted of 60 teeth which were stored in the open environment and salt water, respectively, for a period of 3, 9, and 15 months. DNA was isolated and quantified followed by the amplification of X and Y chromosomes by PCR and compared with the actual gender of the child. The data were analysed using the Shapiro-Wilk test, the independent sample t-test, paired t-test, and the Chi-square test.
The PCR analysis results of Group I showed a more correct interpretation of gender as compared to Group II on storage for a period of 15 months. The PCR analysis results of the Control group showed a 100% accuracy rate as compared to the samples in Groups I and II.
Gender could be effectively determined from the samples evaluated immediately after extraction. But the period of storage and the method of storage conditions affected the quality of isolated DNA and thus decreased the ability of gender determination.
本研究旨在评估使用聚合酶链反应（PCR）从乳牙牙髓组织中确定性别。
选择 140 颗拔出的乳牙。对照组包括 20 颗牙齿，这些牙齿立即进行 DNA 分析。而第 I 组和第 II 组各有 60 颗牙齿，分别在开放环境中和盐水中储存 3、9 和 15 个月。随后，分离并定量 DNA，通过 PCR 扩增 X 和 Y 染色体，并与儿童的实际性别进行比较。使用 Shapiro-Wilk 检验、独立样本 t 检验、配对 t 检验和卡方检验分析数据。
第 I 组在储存 15 个月后，PCR 分析结果比第 II 组更能正确解释性别。与第 I 组和第 II 组相比，对照组的 PCR 分析结果显示出 100%的准确率。
从提取后立即评估的样本中可以有效地确定性别。但是储存时间和储存条件的方法影响了分离 DNA 的质量，从而降低了性别确定的能力。
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