Faculty of Medicine, Department of Plastic, Reconstructive and Aesthetic Surgery, Manisa Celal Bayar University, Manisa, Turkey.
Faculty of Medicine, Department of Histology and Embryology, Niğde Ömer Halisdemir University, Niğde, Turkey.
Biotech Histochem. 2021 Oct;96(7):526-535. doi: 10.1080/10520295.2020.1831066. Epub 2020 Oct 27.
We investigated lateral thoracic and posterior thigh perforator flaps for viability, vascularization, perfusion and apoptosis in a rat model. Wistar albino rats were divided into six groups: lateral thoracic artery perforator flap (LTPF) sham, 3 × 2 cm LTPF, 3 × 6 cm LTPF, posterior thigh perforator flap (PTPF) sham, 3 × 2 cm PTPF, and 3 × 6 cm PTPF. Flap viability was determined on postoperative days 1 and 7. On day 7, flaps were photographed and their viability was measured using two-dimensional planimeter paper. Tissue samples were harvested for examination by histology and immunohistochemistry. Viability differences were statistically significant. Epithelial thickness, vascularity and number of fibroblasts were reduced in the 3 × 6 cm groups. Neovascularization and apoptosis based on molecular tests were not significantly different among groups. Flap size and location are important factors for closure of surgical or traumatic defects. We suggest that for clinical application, wound complications will occur less frequently with perforators that nourish large areas of flaps.
我们研究了胸外侧和股后穿支皮瓣在大鼠模型中的存活、血管化、灌注和细胞凋亡情况。Wistar 白化大鼠分为六组：胸外侧动脉穿支皮瓣（LTPF）假手术组、3×2cm LTPF 组、3×6cm LTPF 组、股后穿支皮瓣（PTPF）假手术组、3×2cm PTPF 组和 3×6cm PTPF 组。术后第 1 天和第 7 天测定皮瓣存活率。第 7 天，对皮瓣进行拍照，并使用二维定标器测量其存活率。采集组织样本进行组织学和免疫组织化学检查。皮瓣存活率差异有统计学意义。3×6cm 组的上皮厚度、血管密度和成纤维细胞数量减少。基于分子检测的新生血管形成和细胞凋亡在各组间无显著差异。皮瓣大小和位置是闭合手术或创伤性缺损的重要因素。我们建议，对于临床应用，营养大面积皮瓣的穿支发生创面并发症的频率将更低。
