Esqueda Adrian, Chen Qiang
The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ, USA.
Methods Mol Biol. 2021;2225:25-38. doi: 10.1007/978-1-0716-1012-1_2.
Various systems exist for the robust production of recombinant proteins. However, only a few systems are optimal for human vaccine protein production. Plant-based transient protein expression systems offer an advantageous alternative to costly mammalian cell culture-based systems and can perform posttranslational modifications due to the presence of an endomembrane system that is largely similar to that of the animal cell. Technological advances in expression vectors for transient expression in the last two decades have produced new plant expression systems with the flexibility and speed that cannot be matched by those based on mammalian or insect cell culture. The rapid and high-level protein production capability of transient expression systems makes them the optimal system to quickly and versatilely develop and produce vaccines against viruses such as 2019-nCoV that have sudden and unpredictable outbreaks. Here, expression of antiviral subunit vaccines in Nicotiana benthamiana plants via transient expression is demonstrated.
存在多种用于高效生产重组蛋白的系统。然而，只有少数系统最适合用于生产人用疫苗蛋白。基于植物的瞬时蛋白表达系统为成本高昂的基于哺乳动物细胞培养的系统提供了一种有利的替代方案，并且由于存在与动物细胞内膜系统大体相似的内膜系统，能够进行翻译后修饰。过去二十年来，用于瞬时表达的表达载体技术取得了进展，产生了具有灵活性和速度的新型植物表达系统，这是基于哺乳动物或昆虫细胞培养的系统所无法比拟的。瞬时表达系统快速且高水平的蛋白生产能力使其成为快速且通用地开发和生产针对如2019 - 新型冠状病毒等具有突然且不可预测爆发的病毒的疫苗的最佳系统。在此，展示了通过瞬时表达在本氏烟草植物中表达抗病毒亚基疫苗。
