Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq.
Anim Biotechnol. 2022 Oct;33(5):801-809. doi: 10.1080/10495398.2020.1837146. Epub 2020 Oct 28.
This study was conducted to assess the role of the mitochondrial 12S rRNA variations in the phylogenetic discrimination between two Iraqi breeds of sheep that differ in geographical distribution. A total of 122 animals (68 Awassi and 54 Hamdani) were included in the study. Direct sequencing of amplicons followed by the construction of a median-joining network and several trees were performed to identify the possible phylogenetic differences between both involved breeds. Genetic diversity, relative frequencies, and analysis of molecular variance (AMOVA) were performed to assess the genetic correlation between both populations. The median-joining network and minimized tree values showed that all observed haplotypes were separated into two groups according to their breed. Comprehensive phylogenetic data revealed only one Asian ancestor for all observed haplotypes. As indicated by AMOVA, the observed diversity was mostly due to between-population variation (1.24836%), while within-population variation (0.91221%) accounted for much less. The currently investigated rRNA amplicons exhibited different mitochondrial manifestations between Awassi and Hamdani breeds. Due to the ability of these 12S rRNA amplicons to mimic the geographical diversity for the currently investigated breeds, it is highly recommended to be used as potent mitochondrial genetic markers among broader ovine sequences.
本研究旨在评估线粒体 12S rRNA 变异在两种地理分布不同的伊拉克绵羊品种之间的系统发育区分中的作用。共有 122 只动物（68 只 Awassi 和 54 只 Hamdani）被纳入研究。对扩增子进行直接测序，然后构建中位数连接网络和几棵树，以确定两种涉及品种之间可能存在的系统发育差异。进行遗传多样性、相对频率和分子方差分析（AMOVA），以评估两个群体之间的遗传相关性。中位数连接网络和最小化树值显示，所有观察到的单倍型根据其品种分为两组。综合系统发育数据表明，所有观察到的单倍型都只有一个亚洲祖先。如 AMOVA 所示，观察到的多样性主要归因于种群间的变异（1.24836%），而种群内的变异（0.91221%）则相对较少。目前研究的 rRNA 扩增子在 Awassi 和 Hamdani 品种之间表现出不同的线粒体表现。由于这些 12S rRNA 扩增子能够模拟目前研究品种的地理多样性，因此强烈建议将其用作更广泛的绵羊序列中的有效线粒体遗传标记。
